The antiviral activity of butanol- and acetic acid-soluble fractions, prepared from the leaves and stems of Phyllanthus orbicularis H.B.K., has been investigated against acyclovir-sensitive or -resistant herpes simplex virus type 1 (HSV-1) strains, using human foreskin fibroblast (HFF) and green ape kidney (Vero) cell lines. Both fractions showed antiviral selectivity indexes (SI) from 10.3 to 22.8, while their extracellular virucidal activities reached SI values ranging from 371 to 1,040. Time-addition experiments suggested that the active compounds present in the studied fractions acted on early steps of the virus replication cycle.

Eighteen coumarins from different species of R u t a collected in the Canary lslands were tested for cytostatic activity. Rutarnarin proved to be strikingly cytotoxic.W e tried to establish the structurelactivity relationship and also to elucidate the mechanism of the activity of this constituent.