To study the effects of sleep deprivation on the pituitary-testis axis physiology, we measured the circulating levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), testosterone (T), androstanedione (A), dihydrotestosterone (DHT), estradiol (E,), and cortisol (C) in eight healthy men as follows: phase I (control), phase II (24-h restless period), phase JII (48-h restless period), and phase N (24-h recovery period). All samples were taken at 8:OO a.m. There was a significant decrease of T, A, DHT, and E, in phase II but no decrease in FHS, LH, PRL, or C. In phase I11 there was no further decrease in any androgen, although E, decreased along with the increase of PRL. In phase N E, and PRL tended to return to baseline values, and the androgens were very similar to the controls. FHS, LH, and C showed no change under the effects of phase III. These data extend the adaptive androgenic response and the association of the role of E, and PRL to restricted or disturbed sleep in men.