The essential oils of plants of the genus Piper have secondary metabolites that have antimicrobial activity related to their chemical composition. The objective of our work was to determine the chemical composition and evaluate the antifungal activity of the aerial part essential oil of P. auritum obtained by hydrodistillation on Fusarium oxysporum and Fusarium equiseti isolated from Capsicum chinense. The antifungal activity was evaluated by direct contact and poisoned food tests, and the minimum inhibitory concentration (MIC50) and maximum radial growth inhibition (MGI) were determined. The identification of oil metabolites was carried out by direct analysis in real time mass spectrometry (DART-MS). By direct contact, the essential oil reached an inhibition of over 40% on Fusarium spp. The 8.4 mg/mL concentration showed the highest inhibition on F. oxysporum (40–60%) and F. equiseti (>50%). The MIC50 was 6 mg/mL for F. oxysporum FCHA-T7 and 9 mg/mL for F. oxysporum FCHJ-T6 and F. equiseti FCHE-T8. DART-MS chemical analysis of the essential oil showed [2M-H]− and [M-H]− adducts of high relative intensity that were mainly attributed to eugenol and thymol/p-cimen-8-ol. The findings found in this study show a fungistatic effect of the essential oil of P. auritum on Fusarium spp.

The objective of this study was to compare the morphology of M. spicata and R. officinalis plants, and the relative abundance quantification, colony-forming units, ribotypes, and biofilm former bacteria under an inorganic fertilizer and the use of vermicompost leachate in the rhizosphere under a closed hydroponic system. In mint (Mentha spicata) plants treated with the vermicompost leachate, growth increase was determined mainly in root length from an average of 38 cm in plants under inorganic fertilizer to 74 cm under vermicompost leachate. In rosemary (Rosmarinus officinalis), no changes were determined between the two treatments. There were differences in the compositions of microbial communities: For R. officinalis, eight ribotypes were identified, seven for inorganic fertilizer and four for vermicompost leachate. For M. spicata, eight ribotypes were identified, three of them exclusive to vermicompost leachate. However, no changes were observed in microbial communities between the two treatments. Otherwise, some changes were observed in the compositions of these communities over time. In both cases, the main found phylum was Firmicutes, with 60% for R. officinalis and 80% for M. spicata represented by the Bacillus genus. In conclusion, the use of vermicompost leachate under the hydroponic system is a viable alternative to achieve an increase in the production of M. spicata, and for both plants (mint and rosemary), the quality of the product and the microbial communities that inhabited them remained unaltered.

Drought is one of the major factors limiting global crop yield. In Mexico, agriculture is expected to be severely affected by drought. The Capsicum genus has several crop species of agricultural importance. In this work, we analysed the Capsicum chinense plant physiological responses and differentially expressed genes under water stress mainly focused on the responses elicited following recovery through repetitive stress. Plants were cultivated in an experimental block. Each block consisted of plants under water deficit and a control group without deficit. Morphometric and functional parameters, and the expression of genes related to resistance to abiotic stresses were measured. Morphological differences were observed. Plants subjected to water deficit showed impaired growth. Nonetheless, in the physiological parameters, no differences were observed between treatments. We selected abiotic stress-related genes that include heat-shock proteins (HSPs), heat-shock factors (HSFs), transcription factors related to abiotic stress (MYB, ETR1, and WRKY), and those associated with biotic and abiotic stress responses (Jar1 and Lox2). HSF, HSP, MYB72, ETR1, Jar1, WRKYa, and Lox2 genes were involved in the response to water-deficit stress in C. chinense plants. In conclusion, our work may improve our understanding of the morphological, physiological, and molecular mechanisms underlying hydric stress response in C. chinense.