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The separation of Petunia sensu Wijsman and Calibrachoa was supported by chloroplast DNA analysis. Two groups in the Calibrachoa were also recognized with a high degree of confidence.

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Nuclear DNA contents over the total range of the genus Petunia sensu Jussieu comprising 20 taxa of Petunia sensu Wijsman (2n 2x 14) and 32 taxa of Calibrachoa (2n 2x 18) were estimated by¯ow cytometry after staining the nuclei with propidium iodide (PI) or 4H ,6-diamidino-2-phenylindole (DAPI). With respect to nuclear DNA content, taxa of Petunia sensu Wijsman seemed to be homogeneous (2C 2 . 60 to 3 . 41 pg), but Calibrachoa taxa were clearly separated into two groups: (1) C. parvi¯ora and C. pygmaea (1 . 56 to 1 . 91 pg); and (2) remaining members of Calibrachoa (2 . 84 to 3 . 26 pg). Taxa of Petunia sensu Wijsman exhibited larger PI/DAPI ratios (relative¯uorescence intensity with PI staining to that with DAPI staining) than Calibrachoa species except C. parvi¯ora and C. pygmaea. This suggests that Petunia sensu Wijsman has nuclear DNA with more adenine-thymine rich regions than Calibrachoa.# 2000 Annals of Botany Company