Qualidade microbiológica da água em locais de pesca artesanal no Rio Santo Antônio como subsídio de monitoramento costeiro no município de Paço do Lumiar-MA Microbiological quality of water in places and artisanal fishing in the Santo Sntônio River as a coastal monitoring subsidy in the municipality of Paço do Lumiar-MA

The aim of study is to feature the microbiological quality of two neotropical fish species from the quilombola area of Maranhão State, Brazil. In order to do so, 21 samples of Hoplerythrinus unitaeniatus and 21 samples of Cichlasoma bimaculatum were captured in flooded environment. Collected fish were euthanized in laboratory environment; muscle fragments were removed for microbiological analyses focused on enumerating molds and yeasts, viable strict and facultative mesophilic microorganisms and coagulase-positive staphylococci; on counting total and thermotolerant coliforms; and on investigating Escherichia coli and Salmonella sp. Microbiological results were compared to the Brazilian legislation, which establishes the list of microbiological standards for food products. Among the assessed fish, 9.52% were classified as non-acceptable for human consumption, based on the Salmonella parameter. Enumerated coagulase-positive staphylococci ranged from < 10 to 3.9 x 104 CFU/g; 9.52% of assessed fish were classified as having intermediate standard for human consumption, whereas 4.76% were classified as non-acceptable for such a purpose. E. coli counting ranged from 3.6 to > 1,100MPN/g; 4.76% of assessed fish were classified as having intermediate standard for human consumption, whereas 4.76% were classified as non-acceptable for such a purpose. Total and thermotolerant coliforms’ counting and the enumeration of viable strict and facultative aerobic microorganisms, as well as of molds and yeasts, have evidenced high microbial population rates; this finding suggests poor hygienic conditions at capture site, contaminated raw material and risk of incidence of enteropathogens. This finding has evidenced imbalance in the investigated environment, as well as compromised aquatic biodiversity.

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O eBook “Ensino de Biologia na atualidade: tendências e perspectivas” tem como objetivo reunir protocolos de prática, estudos de caso, pesquisa científica e de revisão de literatura sobre o ensino de Biologia na atualidade, divulgando pesquisas e relatos de experiências realizadas no Brasil e no Mundo. Visamos disponibilizar essa coletânea gratuitamente para o público interessado que poderá utilizá-lo como base didático-pedagógica para estudantes, professores dos diversos níveis de ensino e demais interessados pela temática. Os capítulos desse E-book constituem a materialização das discussões teóricas e das práticas de Biologia e ensino de Ciências realizadas em sala de aula, em campo e nos laboratórios. Com esta publicação esperamos que professores/as e estudantes percebam a importância de se refletir sobre as novas tendências e perspectivas para fortalecer a Ciência e a Biologia na construção e desconstrução do conhecimento.