The functional effect of platelet membrane glycoprotein Ia (GPIa) gene 807C/T polymorphism in unstable angina pectoris (UAP) pathogenesis was investigated in Chinese Han individuals. Collagen type I-induced platelet aggregation was measured in 33 healthy subjects. Plasma levels of α-granule membrane protein (GMP-140) were measured in 33 healthy subjects and in 35 patients with recent-onset angina at rest within 24 h of hospitalization. Platelet membrane GPIa gene 807C/T polymorphism was determined in all subjects. Lag-time before 30% platelet aggregation was significantly longer in CC genotype than in TC genotype healthy subjects, although there was no significant difference in maximal platelet aggregation between healthy subjects with either genotype. Plasma GMP-140 levels were significantly higher in TC genotype patients compared with CC genotype patients or healthy subjects; a significant difference was also observed between the latter two groups. It was concluded that rapid initiation of collagen-induced platelet aggregation may be associated with the platelet membrane GPIa T807 allele, which may be important in UAP pathogenesis.

ABSTRACT. This study evaluates the relationship between the genotype and milk protein components in goats. Milk samples were collected from cloned goats and normal white goats during different postpartum (or abortion) phases. Two cloned goats, originated from the same somatic line of goat mammary gland epithelial cells, and three sexually reproduced normal white goats with no genetic relationships were used as the control. The goats were phylogenetically analyzed by polymerase chain reaction-restriction fragment length polymorphism. The milk protein components were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The results indicated that despite the genetic fingerprints being identical, the milk protein composition differed between the two cloned goats. The casein content of cloned goat C-50 was significantly higher than that of cloned goat C-4. Conversely, although the genetic fingerprints of the normal white goats N-1, N-2, and N-3 were not identical, the milk protein profiles did not differ significantly in their milk samples (obtained on postpartum day 15, 20, 25, 30, and 150). These results indicated an association between milk protein phenotypes and genetic polymorphisms, epigenetic regulation, and/or non-chromosomal factors. This study extends 16197©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 16196-16203 (2015) Polymorphisms in milk proteins of cloned and normal goats the knowledge of goat milk protein polymorphisms, and provides new strategies for the breeding of high milk-yielding goats.