Huangjiu (Chinese rice wine) is brewed in an open environment, where bacteria play an important role during the fermentation process. In this study, bacterial community structure and composition changes in the fermented mash liquid of mechanized Huangjiu, well‐fermented manual Huangjiu (wines of good qualities), and poorly fermented manual Huangjiu (wines of poor qualities: spoilage, high acidity, low alcohol content) in different fermentation stages from Guyuelongshan Shaoxing Huangjiu company were analyzed via metagenomic sequencing. And bacterial metabolic difference was analyzed via gene prediction of metabolic pathway enzymes. The results showed that the bacterial diversity degree was abundant, and the number of bacterial species in every sample was approximately 200–400. Lactic acid bacteria (LAB) dominated the bacterial community of Huangjiu fermentation, and lactobacillus was predominant species in well‐fermented Huangjiu while Lactobacillus brevis had an absolute dominance in spoilage Huangjiu. Further, gene prediction revealed that transformation of malate to pyruvate and lactate anabolism was more active in mash liquid of well‐fermented manual Huangjiu, while acetate accumulation was stronger in mash liquid of poorly fermented manual Huangjiu, which explained acidity excess reason in poorly fermented Huangjiu at gene level.
Unsignalized intersection driving is challenging for automated vehicles. For safe and efficient performances, the diverse and dynamic behaviors of interacting vehicles should be considered. Based on a game-theoretic framework, a human-like payoff design methodology is proposed for the automated decision at unsignalized intersections. Prospect Theory is introduced to map the objective collision risk to the subjective driver payoffs, and the driving style can be quantified as a tradeoff between safety and speed. To account for the dynamics of interaction, a probabilistic model is further introduced to describe the acceleration tendency of drivers. Simulation results show that the proposed decision algorithm can describe the dynamic process of two-vehicle interaction in limit cases. Statistics of uniformly-sampled cases simulation indicate that the success rate of safe interaction reaches 98%, while the speed efficiency can also be guaranteed. The proposed approach is further applied and validated in four-vehicle interaction scenarios at a four-arm intersection.
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