Apples are susceptible to infection by various pathogens during growth, which induces various leaf diseases and thus affects apple quality and yield. The timely and accurate identification of apple leaf diseases is essential to ensure the high-quality development of the apple industry. In practical applications in orchards, the complex background in which apple leaves are located poses certain difficulties for the identification of leaf diseases. Therefore, this paper suggests a novel approach to identifying and classifying apple leaf diseases in complex backgrounds. First, we used a bilateral filter-based MSRCR algorithm (BF-MSRCR) to pre-process the images, aiming to highlight the color and texture features of leaves and to reduce the difficulty of extracting leaf disease features with subsequent networks. Then, BAM-Net, with ConvNext-T as the backbone network, was designed to achieve an accurate classification of apple leaf diseases. In this network, we used the aggregate coordinate attention mechanism (ACAM) to strengthen the network’s attention to disease feature regions and to suppress the interference of redundant background information. Then, the multi-scale feature refinement module (MFRM) was used to further identify deeper disease features and to improve the network’s ability to discriminate between similar disease features. In our self-made complex background apple leaf disease dataset, the proposed method achieved 95.64% accuracy, 95.62% precision, 95.89% recall, and a 95.25% F1-score. Compared with existing methods, BAM-Net has higher disease recognition accuracy and classification results. It is worth mentioning that BAM-Net still performs well when applied to the task of the leaf disease identification of other crops in the PlantVillage public dataset. This indicates that BAM-Net has good generalization ability. Therefore, the method proposed in this paper can be helpful for apple disease control in modern agriculture, and it also provides a new reference for the disease identification of other crops.
Rice is a crucial food crop, but it is frequently affected by diseases during its growth process. Some of the most common diseases include rice blast, flax leaf spot, and bacterial blight. These diseases are widespread, highly infectious, and cause significant damage, posing a major challenge to agricultural development. The main problems in rice disease classification are as follows: (1) The images of rice diseases that were collected contain noise and blurred edges, which can hinder the network’s ability to accurately extract features of the diseases. (2) The classification of disease images is a challenging task due to the high intra-class diversity and inter-class similarity of rice leaf diseases. This paper proposes the Candy algorithm, an image enhancement technique that utilizes improved Canny operator filtering (the gravitational edge detection algorithm) to emphasize the edge features of rice images and minimize the noise present in the images. Additionally, a new neural network (ICAI-V4) is designed based on the Inception-V4 backbone structure, with a coordinate attention mechanism added to enhance feature capture and overall model performance. The INCV backbone structure incorporates Inception-iv and Reduction-iv structures, with the addition of involution to enhance the network’s feature extraction capabilities from a channel perspective. This enables the network to better classify similar images of rice diseases. To address the issue of neuron death caused by the ReLU activation function and improve model robustness, Leaky ReLU is utilized. Our experiments, conducted using the 10-fold cross-validation method and 10,241 images, show that ICAI-V4 has an average classification accuracy of 95.57%. These results indicate the method’s strong performance and feasibility for rice disease classification in real-life scenarios.
The model species genomes project has played a very important role in the research of human genomes. By comparing and identifying the genomes' information in the different biological evolution stages with the model species genomes, it is favorable to understand deeply the genomes' structure and function of the advanced species , especially the human being , and to reveal the life's essential law. In the paper, the DNA sequences of three model species including C. elegans , S. cerevisiae and A.thaliana are divided into three types: intron, exon,intergenic DNA at first. The bases of each sequence are given number (1, 2,…, N)base by base from the beginning. According to three phases of the codons, we can extract three subsequences which numbers are respectively 3n+1, 3n+2 and 3n+3 (n=0, 1, 2, …,N/3-1).Calculating respectively the probability of four bases in three subsequence we can attain 12 parameters which are regarded as the state parameters of the diversity sources. Each sequence of intron, exon and intergenic DNA is expressed by a 12-dimension eigenvector. All of these sequences' 12-dimension samples are divided into the training sample set and the testing sample set. Then, according to the immune network theory, a immune classifier based on the diversity increment is constructed, which considers the reciprocal of the diversity increment as affinity function and makes the immune network evolve in the direction of identifying the antigens by submitting unceasingly the antigens one by one, which is a record of the training sample set. Finally, the classifier has high performance and its prediction accuracy is up to 85% by test.
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