Exposing body tissue, in vivo, to a magnetic field promotes metabolic alterations in the cell membrane's permeability and in the apoptosis phenomenon. This aim of the study was to investigate magnetic field interactions in the process of tissue repair in rats. Twenty-four male Wistar rats, weighing 200-350 g, were assigned to one of the three different groups: Control (without exposure to the magnetic field), South Pole (with exposure to the South magnetic field), and North Pole (with exposure to the north magnetic field). The intensity of the magnetic field used was 1,600 G. All the animals were anesthetized and immobilized on a surgical board in order to receive circular wounds. The size of the wounds was measured by a milimetric paquimeter. For the histological study, the tissues were fixed in paraffin and colored with hematoxylin and eosin. Wound size data were submitted to one-way analysis of variance (ANOVA) and to the test of Student-Newman-Keuls when appropriated. The results of day 5 (F (2,23):F (3,84); P < 0.05), day 10 (F (2,23):F (8,89); P < 0.05), and day 15 (F (2,23):F (7,88); P < 0.05) revealed a significant reduction between the size of the wounds of both North and South groups when compared to Control group. Our data suggest that chronic exposure to a magnetic field of 1,600 G can accelerate the speed of tissue repair in rats.

No abstract

We analysed the motor behaviour of Wistar rats after 7 days lesion in the left striatum, injected with apomorphine (APO) and stimulated by a continuous magnetic field of 3,200 Gauss. For the behaviour assessment, we utilised the activity cage test and the rotarod test. Sixty-eight male Wistar rats were divided into six groups: control, sham, sham magnetic, lesion, and stimulated South and North Poles. After the experiments, coronal sections of the striatum were taken and stained with Nissl for analysis of the lesion. In the activity cage test for distance (F = 3.19), time of activity (F = 5.46) and crossings (F = 3.31) in all groups, except for the North Pole-stimulated group, we observed a significant increase in these behaviours when compared to the control group. Considering the number of counterclockwise turns, we observed a significant increase in the lesion in the South and North Pole stimulation groups compared with the control group. Highlighting the minor number of counterclockwise turns observed in the North Pole-stimulated group in relation to the South Pole-stimulated and Lesion groups (F = 16.01). The rotarod test revealed a decrease in the time spent in this apparatus for the Lesion group when compared to all other groups (F = 5.46). The morphometric analysis showed a reduction in the number of neurons in the Lesion group in relation to all other groups (F = 5.13). Thus, the results suggest that the static magnetic field north and south promoted a distinct behavioural profile and morphological preservation after 7 days of lesion with quinolinic acid associated with APO.

Our results demonstrate a potential therapeutic application of static magnetic fields for the preservation of motor behavior and neurons in regions analyzed after global cerebral ischemia.

Studies have sought to assess various potential neuroprotective therapeutics in Parkinson's disease. The aim of this study was to evaluate the effects of static magnetic field stimulation 14 days after a 6-Hydroxydopamine (6-OHDA) substantia nigra compacta (SNc) lesion on motor behavior, as assessed by the rotarod (RR) test and brain tissue morphology. Forty male Wistar rats were used and were divided into five groups: control group, sham group (SG), lesion group (LG), lesion north pole group (LNPG) and lesion south pole group (LSPG). In groups with magnetic stimulation, a 3200-gauss magnet was fixed to the skull. After the experiments, the animals were anesthetized for brain perfusion. Coronal sections of the SNc were stained with Nissl. The RR test showed a decrease in the time spent on the apparatus in the LG compared with all groups. The LNPG and LSPG had significant increases in the time spent when compared to the LG. A morphometric analysis revealed a significant reduction in the number of neurons in the LG, LNPG and LSPG in relation to the SG. There were a higher number of neurons in the LNPG and LSPG than the LG, and a higher number of neurons in the LSPG than the LNPG. We observed that the LG, LNPG and LSPG showed a higher number of glial cells than the SG, and the LNPG and LSPG showed a lower number of glial cells than the LG. Our results demonstrate a potential therapeutic use of static magnetic fields for the preservation of motor behavior and brain morphology in the SNc after 14 days with 6-OHDA lesion.