RESUMO(Anatomia e micromorfologia comparada da folha de espécies de Psychotria (Rubiaceae) da Floresta Atlân-tica). O gênero Psychotria compreende aproximadamente 2000 espécies, a maioria arbórea distribuídas em florestas tropicais e subtropicais. Dentro de Rubiaceae, o gênero é bem conhecido pelas espécies produtoras de alcalóides bioativos e taxonomia complexa. Os caracteres anatômicos e micromorfológicos de P. hoffmannseggiana, P. carthagenensis, P. deflexa, P. glaziovii, P. leiocarpa, P. racemosa e P. vellosiana foram investigados e descritos. A variação interespecífica foi avaliada, a fim de contribuir para a sistemática do gêwnero. A análise dos componentes principais (PCA) e a análise de agrupamento através das médias não ponderadas de grupos pareados (UPGMA) indicaram quatro grupos baseados na presença e ausência de algumas características estudadas. Mesofilo dorsiventral, folhas hipostomáticas, estômatos paracíticos, organização do sistema vascular do pecíolo e da nervura principal em arco e presença de feixes acessórios são comuns nas espécies examinadas e são características típicas de Rubiaceae. A presença de cristais estilóides e de alcalóides é considerada característica diagnóstica destas espécies de Psychotria. Outras características usadas para a distinção das espécies o gênero são: o contorno do pecíolo, a ornamentação da cutícula e a presença de tricomas. ABSTRACT(Comparative leaf anatomy and micromorphology of Psychotria species (Rubiaceae) from the Atlantic Rainforest). Th e genus Psychotria comprises approximately 2,000 species that are mostly shrubs distributed in forests of the tropics and subtropics. Within the Rubiaceae, Psychotria is well known for its species that produce bioactive alkaloids and for its taxonomic complexity. Th e anatomical and micromorphological characters of the leaves of P. hoff mannseggiana, P. carthagenensis, P. defl exa, P. glaziovii, P. leiocarpa, P. racemosa, and P. vellosiana were investigated and described to understand the interspecifi c variation of these species, which will contribute to the systematics of the genus. Principal component analysis (PCA) and unweighted pair group method analysis (UPGMA) indicated four groups based on the presence or absence of some of the characters studied. Dorsiventral mesophyll, hypostomatic leaves, paracytic stomata, organization of the vascular system of the petiole and midrib in an arc and the presence of accessory bundles are common in the species examined and are typical characteristics of the Rubiaceae. Th e presence of styloid crystals and alkaloids are considered diagnostic characters of Psychotria. Other characteristics used to separate species in the genus are the petiole contour, cuticle ornamentation, and presence of trichomes. Th e results of the UPGMA cluster analyses indicated that there is similarity between Psychotria (subg. Heteropsychotria) and Palicourea species.

ABSTRACT. Capsicum species are frequently described in terms of genetic divergence, considering morphological, agronomic, and molecular databases. However, descriptions of genetic differences based on anatomical characters are rare. We examined the anatomy and the micromorphology of vegetative and reproductive organs of several Capsicum species. Four Capsicum accessions representing the species C. annuum var. annuum, C. baccatum var. pendulum, C. chinense, and C. frutescens were cultivated in a greenhouse; leaves, fruits and seeds were sampled and their organ structure analyzed by light and scanning electronic microscopy. Molecular accession characterization was made using ISSR markers. Polymorphism was observed among tector trichomes and also in fruit color and shape. High variability among accessions was detected by ISSR markers. Although the species studied Characterization of Capsicum species present a wide morphological and molecular variability, this variability was not reflected in anatomical features.

Libidibia ferrea (LF) is a medicinal plant that holds many pharmacological properties. We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms. Histochemical tests and Gas chromatography of the LPLF were performed to characterize its fatty acids. Acetic acid-induced abdominal constriction, formalin-induced pain, and hot-plate test in mice were employed in the study. In all experiments, aqueous extract or LPLF was administered systemically at the doses of 1, 5, and 10 mg/kg. LF aqueous seed extract and LPLF demonstrated a dose-dependent antinociceptive effect in all tests indicating both peripheral anti-inflammatory and central analgesia properties. Also, the use of atropine (5 mg/kg), naloxone (5 mg/kg) in the abdominal writhing test was able to reverse the antinociceptive effect of the LPLF, indicating that at least one of LF lipids components is responsible for the dose related antinociceptive action in chemical and thermal models of nociception in mice. Together, the present results suggested that Libidibia ferrea induced antinociceptive activity is possibly related to its ability to inhibit opioid, cholinergic receptors, and cyclooxygenase-2 pathway, since its main component, linoleic acid, has been demonstrated to produce such effect in previous studies.

A 6,000 Da peptide, named CaTI, was isolated from Capsicum annuum L. seeds and showed potent inhibitory activity against trypsin and chymotrypsin. The aim of this study was to determine the effect of CaTI on Saccharomyces cerevisiae, Candida albicans, Candida tropicalis and Kluyveromyces marxiannus cells. We observed that CaTI inhibited the growth of S. cerevisiae, K. marxiannus as well as C. albicans and induced cellular agglomeration and the release of cytoplasmic content. No effect on growth was observed in C. tropicalis but morphological changes were noted. In the spot assay, different degrees of sensitivity were shown among the strains and concentrations tested. Scanning electron microscopy showed that S. cerevisiae, K. marxiannus and C. albicans, in the presence of CaTI, exhibited morphological alterations, such as the formation of pseudohyphae, cellular aggregates and elongated forms. We also show that CaTI induces the generation of nitric oxide and interferes in a dose-dependent manner with glucose-stimulated acidification of the medium mediated by H(+)-ATPase of S. cerevisiae cells.

Psychotria is the largest Rubiaceae genus and one of the most abundant in the Atlantic Rainforest of Rio de Janeiro State. The present work aims to characterize and compare the wood of nine of these species. The Psychotria wood were characterized by: slightly distinct growth rings; diffuse porosity; solitary vessels or on radial multiples of 2-6 or clusters of 3-5 vessel elements, with terminal and lateral simple perforation plates and vestured and alternate intervessel and vessel-ray pits; septate fiber-tracheids; and rare axial parenchyma. Although, the wood anatomy of the Psychotria may be considered homogeneous, the statistical analyses, based on qualitative and quantitative features, allowed the segregation of the species and indicated the importance of habit (arboreous or shrubby) on the wood anatomy of the species. It is worth mentioning that the sampling was realized in only one studied site (Ilha Grande), which contributed to the conclusion that the wood characteristics are more related to the specificities of each studied taxon than to with environmental variations.