Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC). We also analyzed the role of Stem Cell Factor (SCF) on MSC proliferation and on ASCs metalloproteinases MMP-2, MMP-9 secretion. Healthy dogs were used as BMMSC donors, and ASC were collected from omentum during elective ovariohysterectomy surgery. Both cell types were cultured in IMDM medium with or without SCF, 10% Dog Serum (DS), and incubated at 38 °C with 5% CO2. Growth of BMMSCs and ASCs was exponential until 25-30 days. Flow citometry of MSCs revealed positive results for CD90 and negative for CD34, CD45 and MCH-II. Genes were evaluated by RT-PCR and metalloproteinases by zymografy. Our findings indicate morphological and immunological similarities as well as expression of genes from both origins on analyzed cells. Furthermore, SCF did not affect proliferation of MSCs, however it up-regulated MMP-2 and MMP-9 secretion in ASCs. These results suggest that metalloproteinases are possibly essential molecules pivoting migration.

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La publicidad institucional de la Dirección General de Tráfico ha cobrado cada vez más fuerza en los últimos años. La tarea histórica de la entidad de disminuir la siniestralidad vial y por consiguiente la mortalidad en accidentes de tráfico constituye un gran reto en la actualidad. Este trabajo se plantea con el fin de extraer una serie de resultados y conclusiones cuantitativas y cualitativas atendiendo a las características y dimensiones del discurso audiovisual que la DGT ha mantenido en sus campañas en el periodo 2011-2019. La acotación temporal responde al periodo de vigencia del segundo Plan Estratégico de Seguridad Vial (2011-2020) que sienta las bases de la actuación de la DGT y supone la hoja de ruta en su misión de disminuir la siniestralidad vial. Específicamente se trata de averiguar cuáles son los factores emocionales y persuasivos que encierran estas campañas y cómo se manifiestan, cuáles son los soportes y mensajes utilizados y qué impacto o alcance tienen en el público espectador. Los resultados muestran que en el discurso audiovisual de la DGT a lo largo de la recopilación de las 74 piezas audiovisuales del periodo 2011-2019 predomina una línea negativa que apuesta por campañas de alto impacto emocional a través de mecanismos de persuasión efectivos.

Immunophenotyping is a widely used method for a precise diagnosis and classification of haematopoietic neoplasia in human beings and also in dogs. The gold standard for cell preparation is density gradient centrifugation of mononuclear cells. Alternatively, another way to separate human leukocytes is carrying out whole blood lysis. The aim of this study was to validate whole blood lysis as an alternative method in clinical veterinary procedures using an immunophenotype panel of leukocytes designed by our group. Flow cytometry study of adult canine leukocytes subset groups, using whole blood lysis or mononuclear cells tested against an array of canine leukocyte antibodies were done. Besides differential white blood cell counts were done. Also immunophenotyping studies in whole blood samples stored at 4 °C for 48 h were performed. The Coefficient Variation values were less than 20%, for most of the comparison. Consistent results were observed in phenotyping canine peripheral blood leukocytes. Stability results indicated that whole blood samples might be stored for 48 h without a significant difference in the data compared to samples processed immediately after blood collection. This study shows that whole blood lysis represents an efficient and quick alternative for canine leukocyte preparation. In addition, samples can be analysed immediately or stored for 48 h without a significant difference between them. This is relevant for veterinary medicine considering the lack of facilities in many laboratories to process samples. Flow cytometry, peripheral blood, leukocyte subsets, long-term stability

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