Summary. The influence of pyridoxine deficiency on chicks fed fat‐free and 10% peanut oil‐diets with and without 1 % cholesterol was studied. Cholesterol: The pyridoxine‐deficient diets caused significantly higher plasma cholesterol than the diets with added pyridoxine. The aorta vliolcsterol showed changes in the same direction. Liver chiolesterol was not, significantly affectd. The pyridoxine‐deficient diets caused lower cholesterol content in the heart than did the supplemented diets. Polyevie fatty acids: With a fat‐free, pyrudixine‐deficient diet a marked content of tetraenoic acid was found in heart and liver, whereas the tritenoic acid was proportionally decreased. Feeding of 10% peanut oil without pyridoxine caused less deposition of tetraenoic in heart and especially in the liver than feeding of 10 % peanut oil with pyridoxine. Addition of 1% cholesterol to the diets resulted in a decrease in the amount of tetraenoic acid in heart and liver.

Dam, Henrik, Gunhild Kristensen, Gunhild Kofoed Nielsen and Ebbe Søndergaard. Influence Of dietary cholesterol, cod liver oil and linseed oil on cholesterol and polyenoic fatty acids in tissues from fasted and non‐fasted chicks. Acta physiol. scand. 1959. 45. 31–42. — The deposition of cholesterol and polyenoic fatty acids after feeding diets with no fat, 10 % cod liver oil or 10 % linseed oil with or without 1 % dietary cholesterol for a 4 week period was determined in various tissues of chicks. About half of the chicks examined had no access to food during the last 16 or 18 hours before they were killed. Data for cholesterol in plasma, aorta, heart, liver, brain and fat tissue and for polyenoic fatty acids in heart, liver, brain and fat are presented.

No abstract

The prescnt communication is a continuation of our previous diets and diets containing 10 % peanut oil were compared with respect to their influence on the cholesterol deposition in certain organs resulting from different levels of dietary cholesterol. This study has now been extended t o comprise several levels of peanut oil and to include the influence on the distribution of polyenoic fatty acids in the tissue. study (DAM, PRANGE and SBNDERGAARD 1955) in which "fat-free" Experimental.One hundred and fourty-four chicks received a, "normal diet" (DAM and S~NDERGAARD 1953 (table 2)) from hatching until the 14th day.Thereafter they were distributed into 16 groups (9 chicks in each) and given diets with varying amounts of peanut oil and cholesterol for 4 weeks.The levels of peanut oil used in the present study were 0, 3, 10 and 20 "lo. The levels of cholesterol were 0, 0.1, 0.3 and 1.0 yo.The composition of the diets into which the various levels of peanut oil and cholesterol were incorporated (instead of the corresponding weight of sucrose) was the same as the "fat-free" diet no. 3, table 1, in the paper by DAM, PRAXCE and SDXDERGAARD (1955). Vitamins

In previous communications (DAM and CHRISTENSEN 1952, CHRISTENSEN, DAM and PRANGE 1952, CHRISTENSEN andDAM 1954) we have described the alimentary production of gallstones and the prevention of galIstone formation in hamsters. We have now tried t o cure the alimentary cholelithiasis in hamst,ers by changing the diet from a lithogenic t o a non-lithogenic type. Experimental.The lithogenic diet was diet no. 295 (CHRISTENSEN, DAM and PRANGE 1952). The non-lithogenic diet (no. 493) contained ground polished rice, Fleischmann yeast type 2019, a small amount of cupric sulfate, a larger amount of Iard than diet 295, and no sucrose. The exact composition of the two diets appears from table 1.Thirty-two hamsters were used for the experiment. They were divided into three groups: A, B and C. Each of groups A and C consisted of 10 animals (5 males and 5 females). Group B consisted of 12 animals (6 males and 6 females). At the beginning of the experiment the hamsters were about 29 days old and weighed about 43 g. All three groups received the lithogenic diet no. 295 for 63 days. Thereafter the animals in group A were killed and autopsied, the gallstones collected, washed in distilled water, dried, weighed, photographed and analyzed for cholesterol by the method of HANEL and DAM (1955). The animals of group B continued on the lithogenic diet for an extra 77 day period, so that when they 0 .-I c e &Jj 4 5 m 10 m 12 m 78 m 89 m 28 f 59 f 62 f 64 f 86 f A 17 m 25 m 40 m 24 m 51 m 58 m 36 f B