Objectives: Pathogenic microbes are causal agents for various types of severe and even lethal infectious diseases. Despite of development in medication, bacterial and fungal infections still persist to be a vital problem in health care. Bacteria and several fungal species have shown resistance to antibiotics used in treatment to current medications. Therefore, it is a considerable field of interest in the design and development of novel compounds with antimicrobial activity. Methods: The compounds bearing a heterocyclic ring play an imperative role among other organic compounds with pharmacological activity used as drugs in human for control and cure of various infections. Thiadiazoles containing nitrogen-sulfur atom as part of their cyclic structure which shown wide-ranging application as structural units of biologically active molecules and are very useful intermediates in Medicinal Chemistry. Results: The effectiveness of the thiadiazole nucleus was established by the drugs currently used for the treatment of various infections. 1,3,4-Thiadiazoles and some of their derivatives are widely studied because of their broad spectrum of pharmacological activities. Conclusion:In the present work, a series of 1,3,4-Thiadiazole derivatives were synthesized by cyclization of a group of various benzaldehyde with thiosemicarbazide in the presence of various reagent like FeCl 3 , HCHO by losing a molecule of water. These derivatives were found to possess prominent antimicrobial activity.

In spite of progress in biotechnology and enzymology, the enzymes have been industrialized in recent years for the mounting up the product development in various arena. The ultimate goal of this study comprises the production and purification the amylase enzyme from the bacterial strain. A powerful amylase producer, Bacillus subtilis ISOLATE-4 was isolated, screened and identified from the soil sample. In order to produce extracellular amylase, various physico-chemical parameters were optimized. During optimization, the maximal production of amylase by the isolate at 48 hrs of incubation in 100 rpm was found to be 6.93U/ml, 5.94U/ml, 6.0U/ml at 45ºC, pH 6 with 1% substrate concentration respectively. Ammonium sulphate fractionation was done for rapid precipitation of the amylase at a concentration of 60% and exposed to dialysis showed the 25% purification fold of an enzyme. The dialyzed product was further subjected to DEAE-Cellulose column chromatography resulted in an increase up to 75% purification fold than crude enzyme. The amylase enzyme might be suitable for the liquefaction of starch, detergent, textile and several additional industrial applications.

A simple, rapid, precise, sensitive and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantitative analysis of Pitolisant in pharmaceutical dosage form. Chromatographic separation of Pitolisant was achieved on Waters Alliance-e2695, by using Waters X-Bridge Phenyl, 150mm x 4.6mm, 3.5µm, column and the mobile phase containing 0.1% OPA& ACNin the ratio of 30:70% v/v. The flow rate was 1.0 ml/min; detection was carried out by absorption at 210nm using a photodiode array detector at ambient temperature. The number of theoretical plates and tailing factor for Pitolisant was NLT 2000 and should not more than 2respectively. %Relative standard deviation of peak area of all measurements always less than 2.0. The proposed method was validated according to ICH guidelines. The method was found to be simple, economical, suitable, precise, accurate & robust method for quantitative analysis of Pitolisant and study of its stability.