Background: Virus infections are presently seen as a major public health problem. Hepatitis C Virus (HCV) is recognized as a “silent killer” because the acute infection has no symptoms, and it develops as a chronic infection that causes hepatocellular carcinoma and liver damage. The World Health Organization (WHO) predicts that between 130-170 million people are estimated to have chronic Hepatitis C. Plants have various phytochemical compounds such as alkaloids and flavonoids that have prominent antiviral effects especially anti-HCV. The current HCV treatment still has limitations related to side effects and can lead to viral resistance. Therefore, it is necessary for the discovery and development of novel anti-HCV drugs for alternative and complementary medicine. Objective: This review intends to evaluate the alkaloids and flavonoids that have the potential to be used against HCV by looking at their classification and their mechanism of action. Methods: Twenty-one articles from 2010 to 2022 obtained from PUBMED database using keywords such as isolated compounds, alkaloids, flavonoids, hepatitis C virus. Results: 21 alkaloids and 37 flavonoids reported active against HCV. Alkaloids include quinoline, quinolizidine and isoquinoline. In addition, flavanone, flavonol, flavone, flavan-3-ol, flavonolignan, anthocyanidin and proanthocyanidin comprise flavonoids. The berberine alkaloids and eriodictyol 7-O-(6′′-caffeoyl)-β-D- glucopyranoside flavonoids had the lowest IC50 with values of 0.49 mM and 0.041 nM. Conclusions: Alkaloids and flavonoids compound had good activity against HCV with various mechanisms. Our results provide information of alkaloids and flavonoids to the researcher for the development of alternative and complementary medicine of hepatitis C.

Efforts to inhibit the transmission of COVID-19 are continuing to this day. One of them is using hand-washing soap to prevent the transmission of this virus. The Faculty of Pharmacy, Airlangga University, seeks to implement SDGs-3 through community service activities to increase community empowerment, especially for the younger generation. The utilization of natural ingredients containing many active compounds has yet to be discovered. The solution is to provide information on the source of the active ingredients for hand washing soap, for example, the Rosella flower, explaining the mechanism of soap in inhibiting the spread of viruses and how to make hand washing soap. The activity was carried out in Gresik with counseling and training methods. The outcome was an increase in participants' understanding, as indicated by an average post-test score of >65, with an increase in score of >10% compared to the pretest. In addition, one product of Rosela flower hand washing soap was also obtained. From this activity, it was concluded that there was an increase in understanding of 28%. It is hoped that this increase in understanding and skills for the younger generation can mobilize the surrounding community so that prevention of the transmission of COVID-19 is more massive and conditions of good health and well-being in society will be achieved.