The changes in the carotenoid pigments of the Capsicum annuum var. lycopersiciforme rubrum during maturation have been investigated quantitatively by means of a HPLC technique. In all of the chromatograms, 40 peaks were detected; 34 carotenoids were identified. The total carotenoid content of the ripe fruits was about 1.3 g/100 g of dry weight, of which capsanthin constituted 37%, zeaxanthin was 8%, cucurbitaxanthin A was 7%, capsorubin constituted 3.2%, and beta-carotene accounted for 9%. The remainder was composed of capsanthin 5,6-epoxide, capsanthin 3,6-epoxide, 5,6-diepikarpoxanthin, violaxanthin, antheraxanthin, beta-cryptoxanthin, and several cis isomers and furanoid oxides. The possible biosynthetic routes for the formation of minor carotenoids containing 3,5,6-trihydroxy-beta-, 3,6-epoxy-beta-, and 6-hydroxy-gamma-end groups are described.
The changes in the carotenoid pigments of Capsicum annuum cv.
Szentesi Kosszarvú during
maturation have been investigated quantitatively by means of a HPLC
technique. In all of the
chromatograms, 56 peaks were detected; 34 carotenoids were identified.
In this study, special
attention is paid to the formation of minor carotenoids with
3,6-epoxy-β- (oxabicyclo[2.2.1]) and
3,5,6-trihydroxy-β- end groups. The possible biosynthetic route
and the identity of capsolutein with
the cucurbitaxanthin A are described.
Keywords: Capsicum annuum; carotenoids; analysis; biosynthesis
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