glycinamide. Dry ammonia was bubbled into a stirred suspension of 3.54 g of the S-benzyl-P-mercaptopropionyl-0-benzyl-L-tyrosyl-Lisoleucyl-r.glutaminyl-r.asparaginyl-S-benzyl-L-cysteinyl-L-prolyl-L-leucylglycyl nitrated resin in 150 ml of anhydrous methanol at 0" for 1.5-2 hr (until the solution was saturated with ammonia). The reaction mixture was stirred overnight at 0-4°.26 The methanol and ammonia were removed under aspirator vacuum. Dimethylformamide (200 ml) was added to the dry residue and the suspension was stirred vigorously for 3 hr. The resin was filtered off and washed three times with dimethylformamide.The solvent was removed from the combined filtrate and washings on a rotary evaporator at below 40". The residue was dissolved in 20 ml of dimethylformamide and 15 ml of distilled water was added gradually with stirring. The turbid solution was allowed to stand in the refrigerator overnight. The precipitate was filtered off, washed with ethanol, and dried in cucuo over KOH pellets; yield, 197 mg, mp 234236".A sample was prepared for analysis by reprecipitating twice from a dimethylformamide-water mixture. The compound was filtered off, washed with ethanol, and dried in L'UCUO, over KOH pellets;mp 238-240", [ c Y ]~~D -37.3" (c 0.6, dimethylformamide).Anal. Calcd for C84H15012NllS2: C, 60.8; H, 6.78; N, 12.2.Found: C,60.9; H,6.78; N, 11.9.Deamino-oxytocin. S-Benzyl-P-mercaptopropionyl-0-benzyl-Ltyrosyl -L-isoleucyl -Lglutaminyl -Lasparaginyl-S-benzyl-L-cysteinyl-L-prolyl-L-leucylglycinamide (1 55 mg) was dissolved in 125 ml of stirred boiling liquid ammonia (distilled from sodium in an all-glass apparatus). A fresh sodium stick was introduced and withdrawn when the blue color pervaded the solution. The sodium stick was momentarily introduced intermittently until the blue color persisted for 30 sec. The ammonia was removed by lyophilization at the water pump and the residue was dissolved in 300 ml of 0.03 z trifluoroacetic acid. The p H of the solution was adjusted to 8.0with ammonium hydroxide and an excess of 0.1 N potassium ferricyanide (4.5 ml) was added to the stirred solution. After 15 min AG 3-X4 resin (chloride form) was added and stirring was continued for 15 min to remove ferrocyanide and excess ferricyanide ions. The resin was removed by filtration, and the solution was lyophilized.The lyophilized residue was dissolved in 20 ml of the upper phase of the solvent system 1-butanol-benzene-3.5 aqueous acetic acid 49 mg (41 z).(25) Ammonolysis conditions as described to us by Dr. Maurice Manning in a personal communication.(26) 0.Abstract: Evidence is put forward which describes the structure and stereochemistry of frenolicin as I.he isolation and preliminary characterization of

No abstract

Ficellomycin is a new basic antibiotic produced by Streptomyces ficellus. Ficellomycin, C13H24N8O3, inhibits the growth of gram-positive bacteria in vitro and is effective in the treatment of experimental Staphylococcus aureus infections in mice. * Taxonomic studies were done by Miss ALMA DIETZ of The Upjohn Company. The organism is designated Streptomyces ficellus UC®5438.

No abstract

No abstract