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SummaryThe application of multiple injections and correlation techniques in capillary zone electrophoresis, a method known for its high detection limits, is described. An experimental electrokinetic injection device, particulary suited for fast and reproducible multiple injections is used in combination with a conventional CZE system. The results show a considerable reduction of the detection limit, in agreement with the experience in the already fully developed correlation chromatographic methods, and in agreement with the theoretical predicted values.
A micromachined injection device is described, which can
be connected to a fused-silica capillary. This allows
injection without interrupting the current, which is an
advantage in CZE and essential in correlation CZE. A
typical property is the excellent connection, with low
dead
volume, between the capillary and the microchip. This
results in very good repeatability of the peak parameters
as is demonstrated by conventional electrokinetic injections. Correlation CZE experiments showed a considerable improvement in S/N ratio at high as well as at low
concentrations. An 8-fold improvement in detection
limit
is achieved. The repeatability of peak height and
peak
area in correlation CZE was even better than obtained with
conventional CZE.
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