The alleviating effects of He Ne laser on damage of nuclear actin filaments organization caused by ultraviolet B (UV B) light were investigated. Wheat (Triticum aestivum L. cv. ML7113) seedlings were irra diated with enhanced UV B (10.08 kJ/(m 2 d)), or He Ne laser (5.43 mW/mm 2 ), or combination of He Ne laser and UV B. The existence, distribution and relative content of F actin in the nucleus were demonstrated by confocal microscopy and flow cytometry after fluorescence labeling with FITC phalloidin. It was shown that the enhanced UV B radiation significantly increased the diffuse fluorescence staining that resulted in bright fragments of F actin being observed in the nucleoplasm. The damage caused by UV B radiation was partially alleviated when the He Ne laser was used: it could be seen that F actin distribution in experiment was similar to that in the control.

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