The leaf oils ofPiper callosumfrom Peruvian Amazon andMichelia montanafrom Assam, India, were prepared by hydrodistillation and analyzed by a combination of GC and GC/MS. Twenty five and thirty components have been identified, representing 96.3 and 100.0% of the respective oils. The major constituents were found to be asaricin (syn. sarisan) (35.9 and 81.8%, respectively) and safrole (20.2 and 13.0%). The oil ofP. callosumcontained in addition eugenyl methyl ether (9.7%) and (E)‒asarone (7.8%), compounds not detected in theM. montanaoil. The identity of the principal compound, an isomer of myristicin, was unequivocally established by13C‒NMR spectrometric techniques, especially long‒range1H–13C correlation.
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