No abstract

A glucose sensor employing ferrocene-bound glucose oxidase was fabricated by the screen printing technique. In order to obtain high GOD activity, glucose oxidase (GOD) was modified with ferrocenecarboxylic acid (fc) as an electron mediator. Simultaneous spectroscopic and electrochemical analyses showed that ferrocene-bound GOD (fc-GOD) displayed an increase in catalytic current. The ferrocene acts as an electron transfer relay between the active center of the enzyme and the gold electrode. The screen printing process was used to print successfully the unique fc-GOD containing ink onto the gold electrode patterned on the glass plate. The fc-GOD containing ink was specially developed to be suitable for the printing apparatus. The printed glucose sensor was shown to offer a stable calibration profile and stable electrochemical properties.