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The effect of benzyl adenine (BA) at 0, 2, 4, 6 and 8 mg l -1 on micropropagation of banana shoot tips was studied. This study also included the morphological responses of banana shoot tips especially with 0 & 6 mg l -1 of BA treatments in relation to some biochemical compositions (total soluble phenols, free amino acids, total soluble sugars, chlorophyll a, b and carotenoids). Growth in 6 mg l -1 of BA resulted in increase in the most morphological parameters compared to the rest treatments. The results showed that 6 mg l -1 of BA treatment significantly increased fresh and dry weights, number of shootlets, shootlet and root length and number of leaves and roots/plantlet as compared without BA. Accumulation of total soluble sugars, free amino acids and chlorophylls was enhanced by 6 mg l -1 of BA while the reverse was true with the rest biochemical compositions (total soluble phenols and carotenoids). The biochemical status and BA treatment at 6 mg l -1 during micropropagation of shoot tips in banana may be important for the development and optimization of strategies for large scale propagation and germplasm conservation.