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We report the first case of a human Burkholderia tropica infection. The patient was a premature neonate who had necrotizing enterocolitis with bowel perforation requiring surgical intervention. The stoma care and difficulties in feeding were a chronic problem. At the age of almost 4 months he developed septicemia due to B. tropica. Three consecutive blood cultures grew this organism. The organism was cleared from the blood after a course of imipenem and resolution of post-operative ileus. Our case suggests that environmental and plant pathogens can cause human infection especially in those in an immunocompromised condition.

All qnr and aac(6')-Ib-cr positive isolates were typed by RAPD.Results: Antibiotic resistant Enterobacteriaceae were cultured from 25/27 (93%) adults, 68/77 (88%) children and 42/100 (42%) neonates. A total of 60, 252 and 97 strains were isolated in each group respectively. ESBL-positive strains were present amongst 67/334 (20%) E. coli, 18/43 (42%) K. pneumoniae and 9/32 (28%) other Enterobacteriaceae. Out of 409 strains of Enterobacteriaceae, 309 (76%) strains were resistant to nalidixic acid, 263 (64%) to gentamicin, 19 (5%) to amikacin, 43 (11%) to piperacillin-tazobactam. qnrA, qnrB, qnrS and aac(6 )-Ib-cr genes were present in 3 (0.7%), 3 (0.7%), 47 (11.5%), and 12 (2.9%) isolates, respectively, which were shown to be unique by RAPD. The MIC50 to ciprofloxacin of qnr and aac( 6)-Ib-cr positive isolates was >0.75 mg/l, the MIC90 >32 mg/l. Conclusion:The carriage rate of drug-resistant Enterobacteriaceae in healthy people in Hochiminh City is extremely high. Moreover, genes encoding transferable quinolones, in particular qnrS, are highly prevalent in these strains.

Plant growth promoting rhizobacteria (PGPR) are strains of naturally occurring soil bacteria that live in close vicinity to the plant’s rhizosphere region which possess the capability to augment host growth. This study was conducted to isolate and identify potential PGPR isolates indigenous to Metroxylon sagu, Rottb. rhizosphere. These potential isolates were characterised based on their beneficial plant growth promoting (PGP) properties and identified by molecular analysis via 16S rDNA sequencing. A total of 18 isolates were successfully isolated, out of which five isolates were tested, and designated as (S1A, S2B, S3A, S3C and S42). Among the five isolates, two isolates (S2B and S3C) were found to produce high levels of indole-3-acetic acid (2.96 μg/mL and 10.31 μg/mL), able to fix nitrogen and show significant activity in phosphate solubilisation. The analysis of their sequences via National Center for Biotechnology Information (NCBI) suggested their close identity towards Lysinibacillus sphaericus and Bacillus thuringiensis. It can be concluded that the isolated PGPR possesses beneficial PGP attributes. It can be implied that the isolated PGPR are potential to be used as inoculant biofertilisers, beneficial for Metroxylon sagu, Rottb. growth. Hence, further studies need to be done to evaluate the effectiveness of the beneficial microbes towards sago seedlings growth, under pot experiment.

Objectives: We aimed to develop and evaluate the efficacy of a health education program for increasing knowledge, changing attitudes and promoting preventive practices to reduce the incidence of RTIs among Malaysian Hajj pilgrims.Methods: A quasi-experimental study was conducted among 132 Hajj pilgrims attending Hajj orientation programme organized by a private Hajj companies. Hajj pilgrims were sequentia lly enrolled and assigned to receive smartphone health education application in the intervent ion group (n = 66) and another different smartphone application on general Hajj process in the control group (n = 66). Data were collected from August 2018 to April 2019 at baseline prior to departure and post-intervention, immediately after return from Saudi Arabia using a validated questionnaire.Results: There was no significant difference with respect to socio-demographic characterist ics, KAP of the respondents in the intervention and control group at baseline. However, there was significant improvement in knowledge in the intervention group compared to the control group, based on time (p = 0.005, ἠ2 = 0.075) and group and time interaction (p = 0.031, ἠ2 = 0.046). Likewise, there was significant improvement in attitude based on time (p = 0.035, ἠ2 = 0.044). Similarly, there was significant main effect in practice based on time (p = <0.001, ἠ2 = 0.155) and interaction of group with time (p = 0.042, ἠ2 = 0.041).International Journal of Human and Health Sciences Supplementary Issue: 2019 Page: 30