This study evaluated the overall performance of 0-to-16-d-old, mixed-sex, Cobb x Cobb broiler chicks when dietary phytase levels were supplemented in excess of industry standards. The experimental diet used consisted of a basal corn-soybean meal diet that contained an analyzed 22.2% CP, 0.88% Ca, a deficient total P (tP) level of 0.46% (phytate P = 0.272%), and calculated ME of 3.123 kcal/g diet on an as-is basis. In addition to a positive control diet [0.70% tP], the dietary phytase levels evaluated were 0, 93.75, 187.5, 350, 750, 1,500, 3,000, 6,000, and 12,000 U/kg of diet. Supplementing phytase from 0 to 12,000 U significantly increased body weight gain from 287 to 515 g/chick, feed intake from 381 to 595 g/chick, gain to feed from 0.755 to 0.866, plasma P from 2.5 to 7.1 mg/100 mL, tibia ash from 26 to 41%, tibia ash weight from 0.200 to 0.601 g/tibia, tP retention from 51 to 80%, phytate P disappearance from 40% to 95%, apparent N retention from 58 to 78%, AMEn from 3,216 to 3,415 kcal/kg diet, and reduced P rickets from 80 to 3%. Using nonlinear regression analysis on log-transformed phytase levels, gain to feed, apparent N retention, and AME, responded linearly with respective R2 values of 0.76, 0.82, and 0.72, whereas body weight gain, feed intake, plasma P, P rickets, tP retention, phytate P disappearance, tibia ash percentage, and tibia ash weight responded quadratically with respective R2 values of 0.93, 0.88, 0.85, 0.84, 0.91, 0.96, 0.96, and 0.98. Few statistical differences existed between response data for broilers consuming the positive control diet or diets containing 1,500 to 12,000 U of phytase (P > 0.05). This finding indicates that broilers consuming a tP-deficient corn-soybean meal diet can achieve maximum performance when phytase is supplemented to 12,000 U/kg diet and that current phytase supplementation levels within the poultry industry may need to be reevaluated.

By means of a central composite rotatable design with dietary calcium levels of 0.63, 0.70, 1.10, 1.50 and 1.67% and total phosphorus levels of 0.53, 0.61, 0.81, 1.01 and 1.09%, practical-type rations were fed for 2 weeks to commercial broiler-type chickens. The design involved three replicates for each rotatable point and 15 replicates for the central point with 10 cockerels per replicate or a total of 390 birds. When the experiment was terminated, the chickens were weighted and killed; one tibia was used to determine bone ash and the other tibia for scoring the incidence and severity of tibial dyschondroplasia. The incidence of tibial dyschondroplasia in chickens fed the various diets ranged from 0 to 37%. A high incidence of tibial dyschondroplasia was associated with diets containing high phosphorus and low calcium levels. Tibial dyschondroplasia score and percentage of bone ash were not correlated. No obvious relationships existed between tibial dyschondroplasia incidence and percentage retention of calcium and phosphorus. A second experiment confirmed the finding that increased dietary calcium levels would reduce the incidence of tibial dyschondroplasia when chickens were fed diets high in phosphorus.

Studies were conducted to determine the effect of dietary supplementation with cholecalciferol (D3), 1,25-dihydroxycholecalciferol [1,25-(OH)2D3], 1alpha-hydroxycholecalciferol (1alpha-OHD3), and 25-hydroxycholecalciferol (25-OHD3) on utilization of phytate P by broiler chickens. Three experiments were conducted with corn-soybean meal type diets with D3 and 1,25-(OH)2D3 being tested in one experiment and 1,25-(OH)2D3, 1alpha-OHD3, and 25-OHD3 being tested in two experiments of exactly the same design. In the first experiment, high levels of D3 (110 microg and 220 microg/kg of diet) increased phytate P utilization, but the increase was not as great as that obtained from 1,25-(OH)2D3 supplementation. In the other two experiments, 1,25-(OH)2D3, D3 and 1alpha-OHD3 were consistently effective in increasing phytate P utilization as measured by plasma Ca and P, incidence of P rickets, bone ash, and retention of Ca, P, and phytate P. Supplementation with 25-OHD3 in general gave smaller and more inconsistent responses to these criteria, indicating some inconsistency in its ability to improve phytate P utilization.

Phytase supplementation over a range of different levels of dietary Ca and nonphytate phosphorus (NPP) was investigated by comparing surface response curves from regression equations generated with (experiment 1) and without (experiment 2) phytase using various performance and bone quality parameters. Cobb x Cobb broiler chicks were raised from 0 to 16 d in 2 experiments using corn-soybean meal based diets. Experiment 1 used a 4 x 4 factorial arrangement with diets formulated to contain combinations of 4 levels of Ca: 0.38, 0.58, 0.78, and 0.98% and 4 levels of NPP: 0.2, 0.3, 0.4, and 0.5%. Experiment 2 used a composite rotatable design in which rations were formulated to contain dietary Ca levels of 0.38, 0.47, 0.68, 0.89, and 0.98% and NPP levels of 0.20, 0.24, 0.35, 0.46, and 0.50%. An extra point was included in the design to contain the lowest Ca and lowest NPP levels (0.38% Ca and 0.20% NPP). All combinations of Ca and NPP were fed with 657 phytase units/kg Natuphos 5000 phytase, plus 4 combinations (0.38% Ca and 0.20% NPP, 0.47% Ca and 0.24% NPP, 0.68% Ca and 0.35% NPP, and 0.89% Ca and 0.46% NPP) were fed without phytase to determine the suitability of comparing multiple regression response surfaces for particular variables among experiments. Comparison of surfaces, with and without phytase, showed that growth and bone quality responses to phytase were greatest at low NPP levels and high Ca levels, and these decreased when the Ca level was reduced or when the NPP level was increased. A third experiment confirmed that phytase elicits a greater response at higher Ca levels and lower NPP levels (0.86% Ca and 0.20% NPP) versus low Ca levels and low NPP levels (0.47% Ca and 0.24% NPP). The data demonstrated why it is impossible to determine a single NPP equivalency value for phytase supplements.

Several excellent reviews regarding nutrition and skeletal disorders have appeared in the last 20 yr. This review will cover several areas of vitamin D research, the area of feed deprivation, and bone abnormalities, because there has been considerable interest in these areas during the past 10 yr. Studies indicate that the quantitative requirement for cholecalciferol (D3) for broiler chickens is much greater than previously thought. Ascorbic acid may play a role in stimulating 1-hydroxylation of 25-hydroxycholecalciferol [25-(OH)D3], but the evidence is not clear under exactly what conditions this relationship is important in practical prevention of tibial dyschondroplasia. Studies indicate that dietary supplementation with 1,25-dihydroxycholecalciferol [1,25(OH)2D3] will reduce the incidence of tibial dyschondroplasia in three different strains of broilers bred to develop a high incidence of the disease. But it did not prevent the disease totally in the strains, unless high enough levels of 1,25-(OH)2D3 were fed to reduce growth rate. These studies indicate that these high tibial dyschondroplasia strains have a defect(s) in vitamin D metabolism. Studies continue to elucidate the role of ultraviolet light in preventing leg abnormalities. Only a few studies have been conducted on the efficacy of various vitamin D3 derivatives to prevent tibial dyschondroplasia. Feed deprivation continues to be an intriguing method of preventing tibial dyschondroplasia, and examination of exactly how this prevents the bone abnormality could open avenues for explaining the disease.