Development of pre-harvest sprouting (PHS) tolerant varieties is imperative to minimize yield losses due to viviparous germination. F2 seeds derived from the crosses DGGV-2 x Pant Moong-1 were irradiated with 60 kR gamma rays for the creation of desirable variability. In F2M2 generation, segregants with small pod beak and angle, thick pod wall, hard seededness and higher epicuticular wax and lignin content were isolated. Desirable mutants conferring tolerance to PHS were selected from 206 progenies consisting of 4812 plants. During the subsequent selfing generations the desirable plants with less than five percent PHS were stringently characterized by morphological and biochemical indicators. Due to incessant heavy rain fall (462.2mm) during pod development and maturity coupled with high humidity weather was conducive for viviparous germination, resulting in higher PHS. Correlation analysis between pre harvest sprouting and biochemical and morphological parameters revealed that negative correlation of PHS was observed for epicuticular wax (r = -0.983), phenol (r = -0.892), lignin content (r = -0.981) and hard seed percentage (r = -0.942). Significant negative correlation was observed for pod wall thickness (r = -0.570). Pod beak length recorded highly significant positive correlation (r = 0.911) to PHS. Genotypes with inherent tolerance to seed shattering hold a promise to minimize yield losses due to viviparous germination.
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