The present investigation was conducted with the F3 breeding lines derived from three crosses viz., DGGV-7 × V-02-709, DGGV-7 × V-02-802, DGGV-2 × SML-1815 along with their parents used as checks. The progeny lines were evaluated for estimation of genotypic coefficient of variation (GCV), phenotypic coefficient of variation (PCV), heritability and genetic advance. The correlation study was undertaken among the ten selected quantitative characters. The analysis of variance showed, the progeny lines derived from the cross DGGV-7 × V-02-709, recoded very high significant variation for the characters like the number of branches per plant (2.988), pod length (2.363) and seed yield per plant (13.007g). The 100 seed weight (4.7g) was observed in the cross derivative of DGGV-2 × SML-1815 with high heritability 73.26 per cent and moderate genetic advance under mean (19.65). The breeding lines of DGGV-7 × V-02-709 have recorded mean seed yield 3.7 g with the range of 3.22 to 4.65 g and the PCV (17.35%), GCV (14.23%) was moderate with high heritability (72.12%) coupled with moderate genetic advance over mean (17.45%). There is a positive significant correlation was observed between plant height, number of clusters per plant, number of pods per plant, number of seeds per pod and seed yield per plant. Therefore, these characters should give higher priority at the time of selection for improvement of yield in greengram.
Background: Mungbean yellow mosaic virus disease is the most devastating disease on Mungbean production. The virus is transmitted by whitefly and can cause yield losses from 75 to 100 per cent. The development of mungbean cultivars resistant to both virus and its vector is considered as one of the most desirable means of managing the disease as it is environmentally safe and highly efficient. The selection of resistant genotypes in conventional methods is complex and time consuming. Hence, the use of molecular markers linked with resistance genes is powerful as it hastens the breeding programmes. The current study was aimed to develop mapping population and to validate molecular markers associated with Mungbean yellow mosaic virus (MYMV).
Methods: The present investigation was carried out with 260 F2 individuals that were derived from crossing DGGV-2 and IPM 2-14 during Kharif-2017 at Main Agril Research Station, UAS, Dharwad. Hybrid seeds of this cross were harvested individually and sown during rabi 2017 along with the two parents, as checks for distinguishing the true hybrids. Hybridity of F1s was confirmed through molecular marker analysis and the true F1s were selfed to raise the F2 generation.
Result: Of the 24 previously reported simple sequence repeat markers used for detecting the polymorphism, two markers viz., CEDG305 and CEDG115 were found to be polymorphic between DGGV-2 and IPM-2-14. Two hundred and sixty F2 plants segregated in the ratio of 3 S:1 R (202 susceptible: 58 resistant) as phenotypic and 1: 2 :1 as genotypic ratio implying that single recessive gene controlled resistance. Single marker analysis revealed that the molecular markers CEDG305 and CEDG115 were associated with MYMV resistance with a phenotypic variance of 24.5 and 10.3 per cent respectively.
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