Blood Disease Bacterium (BDB) and Fusarium oxysporum f.sp. cubense (FOC) is a couple wilt pathogen of banana. These pathogens are the most important constraint in cultivation of banana in Indonesia. In the integrated control strategy of the disease, the use of healthy seedlings produced from tissue culture technique is recommended. The seedling produced by tissue culture technique however leads to lower vigor and susceptibility to the disease due to the aseptic work in vitro causing the beneficial bacterial endophytic to be eliminated. Therefore, the utility of the beneficial endophytic bacteria should be studied for recovering the vigor and resistance of the seedling. Three isolates of endophytic Bacillus (B04, B05, B10) have been effective as growth promoter of microplantlet and antagonist of BDB and FOC in vitro. Here then, this article reports the study results of the vigor of the plantlet (treated microplantlet by filtrate or cell suspension of the Bacillus) after 3 months in acclimatization. The results were similar to the previous results on microplantlet in vitro, that Bacillus isolates B04, B05, and B10 were capable of promoting the growth and inducing the resistance to wilt pathogens on banana plantlets. The treatments with bacterial cell inoculums were more effective than those bacterial filtrate. Isolate B10 was most potential followed by B05 and B04 respectively.
<p>Fusarium oxysporum f. sp.cepae (FOCe) causing basal rot of garlic is the main obstacle in improving garlic production. The presence of root knot nematode (Meloidogynespp.) is thought to cause an increase in the disease was caused by FOCe. This research aimed to study the effect of the presence of Meloidogyne in increasing the disease severity of basal rot of garlic caused by FOCe and interactions between them. The research was conducted through surveys and experimental procedures. The survey was conducted to obtain preliminary data on the relationship between the disease severity of basal rot of garlic and the infection of Meloidogyne in soil. Experimental study were prepared using a complete randomized block design, with two factors consisting of three levels inoculum density. The first factor was FOCe and the second factor was Meloidogyne spp. The results showed that the presence of Meloidogyne could increase the disease severity of basal rot of garlic</p>
<p>Basal rot was an important disease in garlic caused by <em>Fusarium oxysporum f. sp. cepae </em>(FOCe) and a major obstacle in garlic cultivation. Root knot nematodes (<em>Meloidogyne</em>) is endoparasitic nematodes causing plants become more susceptible to fungal pathogen infection. This research aimed to study the effect of double inoculation FOCe and Meloidogyne, and current inoculation against disease severity of basal root. The research was conducted through experimental procedures and prepared based on a randomized complete block design, with two factors consisting of three levels inoculation time. The first factor was FOCe and the second factor was Meloidogyne. The results showed that Meloidogyne presence could increase the disease severity of basal rot of garlic. Garlic was inoculated at 35 days after planting (dap) by Meloidogyne and FOCe cause the most severe disease of basal rot.</p>
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