Vigor of Plantlet from Microplantlet Treated by Filtrate and Cell Suspension of Some Isolates of Bacillus and Resistance to Banana Wilt Pathogen after Acclimatization

Abstract

Blood Disease Bacterium (BDB) and Fusarium oxysporum f.sp. cubense (FOC) is a couple wilt pathogen of banana. These pathogens are the most important constraint in cultivation of banana in Indonesia. In the integrated control strategy of the disease, the use of healthy seedlings produced from tissue culture technique is recommended. The seedling produced by tissue culture technique however leads to lower vigor and susceptibility to the disease due to the aseptic work in vitro causing the beneficial bacterial endophytic to be eliminated. Therefore, the utility of the beneficial endophytic bacteria should be studied for recovering the vigor and resistance of the seedling. Three isolates of endophytic Bacillus (B04, B05, B10) have been effective as growth promoter of microplantlet and antagonist of BDB and FOC in vitro. Here then, this article reports the study results of the vigor of the plantlet (treated microplantlet by filtrate or cell suspension of the Bacillus) after 3 months in acclimatization. The results were similar to the previous results on microplantlet in vitro, that Bacillus isolates B04, B05, and B10 were capable of promoting the growth and inducing the resistance to wilt pathogens on banana plantlets. The treatments with bacterial cell inoculums were more effective than those bacterial filtrate. Isolate B10 was most potential followed by B05 and B04 respectively.

The antagonistic mechanism of rhizosphere microbes and endophytes on the interaction between banana and Fusarium oxysporum f. sp. cubense

“…The good ability of B subtilis and P. fluorescens, to control the rhizome rot of banana has also been reported by Nagaraj et al [82]. Wiyono and Widono [83] reported that Bacillus species can promote growth as well as induction of resistance to wilt pathogens on tissue culture banana plantlets. Application of plant growth-promoting rihzobacteria (PGPR) on tissue culture banana resulted in high growth rate and vigour in plantlets [84].…”

Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum f. sp. cubense race 1

“…It has been shown that certain bacterial strains under controlled conditions suppress the growth of F. oxysorum (Ho et al, 2015;Sekhar & Thomas, 2015;Simonetti et al, 2018;Zacky & Tiny, 2013). Within these bacterial groups several strains of the genus Bacillus have been described (Wiyono & Widono, 2013;Xue et al, 2015). This genus, in particular, has been widely studied as a biological control agent due to its multiple mechanisms of antagonistic action such as antibiosis, competition, and the induction of systemic resistance in plants (Chowdhury representar una alternativa más sostenible desde el punto de vista económico y ecológico.…”

Biohardening of Tissue Cultured Banana Plantlets of cv. Ney Poovan for the Management of Fusarium wilt of Banana with Bacillus amyloliquefaciens (VB7) Triggers Defence Gene Products and Growth Promotion

“…At present disease free quality banana planting material is commercially propagated through tissue culture [6], but in the process of in vitro propagation most of the beneficial endophytic bacteria are also eliminated. Hence, the tissue cultured plantlets are highly and easily susceptible to Fusarium wilt in early stages if proper control measures are not taken up as compared to conventional planting material [7].…”