No abstract

No abstract

The essential oil obtained by hydrodistillation of the flowers of Rhanterium adpressum Coss. & Durieu was analyzed using GC and GC-MS. The essential oil was very rich in monoterpene compounds. The major components identified were the monoterpene hydrocarbons: camphene (21.8%), myrcene (19.3%) and αpinene (17.4%). Other compounds, including limonene, β-pinene and terpinol-4-ol, were present in low content (4-6%). The composition of the fatty acids in the lipid extract obtained from the flowers was also investigated by GC and GC-MS. The main fatty acids identified were palmitic (47.4%), oleic (12.9%) and stearic acids (10.6%). The total phenolic contents and the antioxidant activities were also evaluated for both extracts. The total phenolic contents were determined using the Folin-Ciocalteu reagent and the antioxidant activities were measured using three different assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity, FRAP (ferric reducing antioxidant potential) and a molybdenum assay. As a result of these tests, the lipid extract exhibited the highest antioxidant activities in comparison with the essential oil extract.

No abstract

Background: This paper focuses on one of the most recurrent hypotheses for the antidiabetic effect of medicinal plants by inhibiting the alpha -glucosidase Objective: In the present study aqueous and hydro -alcoholic extracts of eighteen medicinal plants, collected in the town of Laghouat in the steppe region of Algeria, and which are used in the treatment of diabetes, were examined for α-glucosidase inhibition using an in vitro model. Also total phenol content and antioxidant activity of the extracts was investigated. Methods: The extracts were prepared using distilled water and hydro -alcoholic solvent (methanol/water). The amount of total phenolics and flavonoids in the samples was determined with the Folin-Ciocalteu reagent and with aluminum chloride respectively. To identify plants with inhibitory capacities, we have studied the effects of ours extracts on the kinetics catalyzed of α-glucosidase an enzymes belonging to the class of hydrolase responsible for the digestion and we have subjected our extracts to inhibition assay to determine the inhibition percentage for each extract. Finally, we assessed the potential antioxydant of the best inhibitory plants with the DPPH test. Results: The major plants were found to inhibit enzymatic activity of α-glucosidase and the values of the IC50 constants have been determined for the best inhibitory plants common in both extraction methods (Cistus SP, Salvia officinalis and Zygophylum album), with values ranged from 0.38 to 28.95 mg/ml. The evaluation of antioxidant activity of this plants using DPPH test, shows that the phenolic extract of Salvia officinalis exhibit good antioxidant capacity (8.46 μg/ml) comparatively to the antioxidants taken as reference (BHA, Gallic acid, Rutin, Trolox and Vitamin C). Conclusion: This study is the first report on potential inhibition of these plants extracts on the digestive enzyme, α-glucosidase. The obtained results support the traditionally use of a number of the analyzed species in the in the management of diabetes.