A halophilic archaeal strain, designated CBA1105(T), was isolated from non-purified solar salt. Strain CBA1105(T) was found to have three 16S rRNA genes, rrnA, rrnB and rrnC; similarities between the 16S rRNA gene sequences were 99.5-99.7 %. The phylogenetic analysis of the 16S rRNA gene sequences showed that strain CBA1105(T) forms a distinct clade with the strains of the closely related genera, Halorientalis and Halorhabdus, with similarities of 94.2 % and 93.9-94.2 %, respectively. Multilocus sequence analysis confirmed that strain CBA1105(T) is closely related to the genus Halorhabdus or Halorientalis. Growth of the strain was observed in 15-30 % NaCl (w/v; optimum 20 %), at 30-45 °C (optimum 37 °C) and pH 7.0-8.0 (optimum pH 7.0) and with 0-0.5 M MgCl2·6H2O (optimum 0.05-0.2 M). The cells of the strain were observed to be Gram-stain negative and pleomorphic with coccoid or ovoid-shape. The cells lysed in distilled water. Tweens 20, 40 and 80 were found to be hydrolysed but starch, casein and gelatine were not. The cells were unable to reduce nitrate under aerobic conditions. Assays for indole formation and urease activity were negative and no growth was observed under anaerobic conditions. Cells were found to be able to utilize L-glutamate, D-glucose, L-maltose, D-mannose and sucrose as sole carbon sources. The polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, unidentified glycolipids and an unidentified phospholipid. The G+C content of strain CBA1105(T) was determined to be 66.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that the strain represents a novel species of a novel genus within the family Halobacteriaceae, for which the name Halapricum salinum is proposed with CBA1105(T) (= KCTC 4202(T) = JCM 19729(T)) as the type strain.

An anaerobic, rod-shaped, hyperthermophilic and acidophilic crenarchaeon, designated strain CBA1501 T , was isolated from solfataric soil of the Mayon volcano in the Republic of the Philippines. Phylogenetic analysis showed that strain CBA1501 T is affiliated with the genus Vulcanisaeta in the phylum Crenarchaeota. DNA sequence similarities between the 16S rRNA gene of strain CBA1501 T and those of Vulcanisaeta distributa IC-017 T and Vulcanisaeta souniana IC-059 T were 98.5 and 97.4 %, respectively. Strain CBA1501 T grew between 75-90 6C, over a pH range of 4.0-6.0 and in the presence of 0-1.0 % (w/v) NaCl, with optimal growth occurring at 85 6C, pH 5.0, and with 0 % (w/v) NaCl. Fumarate, malate, oxidized glutathione, sulfur and thiosulfate were used as final electron acceptors, but FeCl 3 , nitrate and sulfate were not. The DNA G+C content of strain CBA1501 T was 43

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Strain CBA3205T is a Gram-stain-negative, non-motile and rod-shaped bacterium that was isolated from the seashore sand of Jeju Island in South Korea. Based on the phylogenetic analysis, the most closed related species was Croceitalea eckloniae DOKDO 025 T , with 94.8 % sequence similarity for the 16S rRNA gene. Strain CBA3205T was observed to grow optimally at 25-30 8C and at pH 8.5 in the presence of 2-3 % (w/v) NaCl. The major fatty acids of strain CBA3205 T were iso-C 15 : 0 , iso-C 15 : 1 G, and iso-C 17 : 0 3-OH. The major respiratory quinone was MK-6 and the major polar lipids were two unidentified amino-groupcontaining phospholipids and an unidentified polar lipid. The G+C content of the genomic DNA of strain CBA3205 T was 62.5 mol%. Based on the phenotypic, genotypic and phylogenetic analyses, strain CBA3205 T was considered to be a novel species belonging to the genus