BackgroundVitabridC12 is newly developed and composed of vitamin C and Vitabrid (lamellar, hydrated zinc oxide).ObjectiveIn this study, we aimed to investigate the effects of VitabridC12 on psoriasis and atopic dermatitis.MethodsMice with imiquimod-induced psoriasis or Dermatophagoides farinae-induced atopic dermatitis were applied with VitabridC12. The effects of VitabridC12 were evaluated by clinical features, histology, and immunologic features by examining cytokines and chemokines.ResultsIn psoriasis model, VitabridC12 decreased epidermal thickness and reduced inflammatory cell infiltration. In atopic dermatitis model, VitabridC12 decreased dermal infiltration of inflammatory cells, epidermal hyperplasia, and hyperkeratosis. VitabridC12 reduced the expression levels of proinflammatory mediators such as interleukin (IL)-1β, IL-6, IL-8, IL-17A, IL-22, tumor necrosis factor-α, CXCL1, CCL17, and CCL20 as well as COX-2 in imiquimod-induced psoriatic skin lesions. Likewise, VitabridC12 reduced the expression levels of IL-4, IL-5, IL-13, thymic stromal lymphopoietin, and CCL4 in D. farinae-induced skin lesions, and decreased the serum immunoglobulin E level in the atopic dermatitis mouse model. Particularly, the VitabridC12-treated mice showed downregulated expressions of mitogen-activated protein kinase (MAPK), including extracellular signal-regulated kinase (ERK), p38, and MAPK/ERK kinase, as well as inhibited phosphorylation of nuclear factor-κB p65.ConclusionTaken together, these findings indicate that VitabridC12 exhibits anti-inflammatory activities and is a promising candidate as a treatment option for psoriasis or atopic dermatitis.

The influence of mesenchymal stem cells (MSCs) on keratinocytes in altered microenvironments is poorly understood. Here, we cocultured umbilical cord blood-derived MSCs with normal human epidermal keratinocytes to evaluate their paracrine effect in the presence of high extracellular calcium (Ca ) concentration. High Ca environment to keratinocytes can disrupt normal skin barrier function due to abnormal/premature differentiation of keratinocytes. Surprisingly, we found that MSCs suppress both proliferation and differentiation of keratinocytes under a high Ca environment in transforming growth factors β1 (TGFβ1)-dependent manner. Furthermore, we determined that MSCs can regulate the mitogen-activated protein kinases, phosphatidylinositol 3-kinase/protein kinase B, and protein kinase C pathways in Ca -induced differentiated keratinocytes. Knockdown of TGFβ1 from MSCs results in decreased suppression of differentiation with significantly increased proliferation of keratinocytes compared with control MSCs. MSCs-derived TGFβ1 further induced growth inhibition of keratinocyte in high extracellular Ca environment as analyzed by a decrease in DNA synthesis, accumulation of phosphorylated retinoblastoma protein, cdc2, and increased mRNA level of p21, and independent of TGFβ1/SMAD pathway. Taken together, we found that MSCs-derived TGFβ1 is a critical regulator of keratinocyte function, and involves multiple proximal signaling cascades. Stem Cells 2017;35:1592-1602.

Notre étude a pour objectif d’examiner la possibilité d’appliquer, dans le domaine de l’enseignement de la traduction, le SILL (Strategy Inventory for Language Learning), questionnaire destiné à mettre en évidence les types de stratégies mis en oeuvre par des apprenants en langues, instrument largement utilisé lors de l’apprentissage linguistique. Pour ce faire, nous avons procédé à une analyse du questionnaire SILL rempli par des étudiants ayant assisté aux cours de traduction. Celle-ci a effectivement montré une corrélation entre les compétences des étudiants et leurs choix de stratégies : les étudiants compétents ont plus eu recours aux stratégies de la mémoire, cognitives, et de compensation, tandis que les moins compétents se sont plutôt tournés vers les stratégies métacognitives, sociales et de l’affection. Une analyse plus approfondie a d’ailleurs été réalisée pour identifier les stratégies les plus efficaces employées pendant l’acte de traduire. En conclusion, le SILL apparaît comme un outil utile servant à analyser les stratégies des étudiants dans leur tâche de traduction, fournissant ainsi des informations fiables en vue d’améliorer l’efficacité des cours de traduction.