This study aims to demonstrate the potential use of reusable electrochemical sensor for detecting pork DNA in solution. The approach was based on electrochemical principle in which the electrostatic interaction between DNA and redox species will generate detectable signal upon introduction of electrical charge. In this study, Ruthenium Hexaamine (RuHex) was used as the redox species and result was based on the output current. Coupled with highly specific Polymerase Chain Reaction (PCR) primers designed for pork DNA, this study has successfully demonstrated the reliability of proposed novel detection approach that utilized reusable electrochemical sensor and can potentially be developed into a rapid detection tool for halal and kosher food industry.