This study was conducted to determine the effects of the fermentation duration of coconut dregs (CD) by Saccharomyces cerevisiae and the addition of ammonium sulfate on the growth performance, feed digestibility, carcass, and digestive organ developments. A finely ground CD was autoclaved at 20 psi for 20 minutes and added distilled water to meet 80% moisture content. The autoclaved substrate was added with different concentrations of ammonium sulfate and fermented with Saccharomyces cerevisiae to produce Saccharomyces cerevisiae-fermented CD. A total of 192 day-oldunsexed Cobb broiler chicks were used and kept for 6 weeks. The birds were fed experimental diets ad-libitum. The experimental diets were produced by two durations of fermentation (5 days and 7 days) and three levels of ammonium sulfate (0%, 0.2%, and 0.4%) in 4 replicates. The experimental diets were offered ad-libitum and water were available at all times. Fermentation decreased lipid and crude fiber content of CD and the addition of ammonium sulfate increased protein content and amino acid concentration of CD. The bodyweight gain of birds increased when the CD was fermented for 5 days and with the addition of 0.2% ammonium sulfate. Dry matter digestibility and protein digestibility were improved when CD was added with 0.2% ammonium sulfate. In conclusion, fermenting CD for 5 days increased body weight gain and the addition of 0.2% ammonium sulfate improved the feeding value of the diet and growth of birds.

Cocoa pod extract ((Theobroma cacao L.) has antioxidant and antimicrobial activity that has the potential as a natural food preservative. However, in its use the cocoa fruit skin extract has a disadvantage because the short shelf time and its application to food are limited, efforts are needed to prevent damage and extend shelf life, one of the efforts that can be done is by encapsulating the extract.This study aims to determine the antibacterial activity and antioxidant encapsulation of cocoa peel extract, this study begins with the extraction of cocoa pods with ethanol solvent by comparing cocoa pods : solvent 1: 4 The skin of cacao cocoa fruit used is yellow harvested cocoa fruit, then chopped and dried to form flour.The sample is extracted by maceration with ethanol solvent Antioxidant test is done by DPPH method, while antibacterial test is carried out by the well diffusion method. This study used a completely randomized design method (CRD) with 5 treatments using a maltodextrin concentration of 20% (M1); 30% (M2); 40% (M3); 50% (M4) and 60% (M5). The results showed that the treatment gave the highest yield in the treatment of 60% maltodextrin concentration (M5), while the highest antioxidant activity was obtained in the treatment of 20% maltodextrin (M1) with IC50 75.98 µg / mL and the treatment with the lowest antioxidant activity was obtained at treatment of 60% maltodextrin concentration (M5) with IC50 value 114.89 µg / mL. While for the antimicrobial activity also obtained with the same results, namely treatment of 20% (M1) obtained a higher inhibition diameter compared to treatment at 30%; 40%; 50% and 60% for all types of bacteria. The inhibition diameter in the treatment of the concentration of maltodextrin 20% (M1) for E. coli bacteria is between 4.12 mm - 10.95 mm, Salmonella sp is 2.85 mm - 8 , 25 mm and for Staphylococcusaureus of 5.15 mm - 13.90 mm and the lowest inhibition diameter was obtained in the treatment of 60% maltodextrin concentration (M5) for E. coli bacteria of between 2.0 mm - 4.79 mm, Salmonella sp of 1.15 mm - 4.35 mm and for Staphylococcusaureusat 2.76 mm - 5.17 mm.This study concluded that the encapsulation of cocoa peel extract using 20% maltodextrin had the highest antioxidant and antimicrobial activity when compared with other treatments namely 30% concentration; 40%; 50% and 60% but for the treatment of 20% and 30% there is no difference. Ethanol extract of cocoa pods can be made in the form of encapsulates which are very likely to be used as natural preservatives.

Essential oils from the distillation of clove leaves could act as anti-bacterial and anti-fungal agentsforeither food or feed. The purpose of this study was to identify chemical compounds and antioxidant content of clove leaves essential oils. The material used was clove leaves essential oil obtained by soxlet extraction. Clove leaves were taken from trees of over 10 years old in Palu, Central Sulawesi. Chemical compounds of the sample were analyzed by GC-MS method. Each peak that appeared on the chromatogram was identified by comparing to the standard. While the antioxidant analysis was carried out by using the DPPH (IC50) of spectrophotometry method. The results showed that the total essential oils content of clove leaves was 3,957% and there were 6 chemical compounds identified, i.e. caryophyllen (0,96%); phenol, 2-methoxy (guaiacol) (4,16%); phenol,2-methoxy-4-methyl (9.79%); phenol, 4-ethyl-2-methoxy (1.5%); phenol 2-methoxy-4-propyl (3.87%); phenol, 2-methoxy-4-(2-prophenyl) andEugenol (79.72%). While the results of antioxidant identification based on DPPH and IC50 values before purification were 0.597 and 74.358, and the respective values after purification were 0.597 and 42.255. In conclusion, the antioxidant content of clove leaves essential oils after purification is higher than before purification and it contains 3.957% essential oil.

Minyak atsiri daun cengkeh mengandung 79,72% eugenol yang merupakan antimikroba, antibiotik dan antioksidan. Penambahan eugenol dalam pakan broiler diharapkan dapat memperbaiki efisiensi penggunaan pakan. Penelitian ini mengevaluasi penggunaan eugenol daun cengkeh dalam pakan yang terdiri atas 5 perlakuan dengan 4 ulangan. Setiap unit percobaan tersebut digunakan 10 ekor ayam pedaging umur 1 minggu dengan berat badan awal 142,25 ± 7,75 g/ekor selama 5 minggu (umur ayam 42 hari), sehingga total ayam yang digunakan dalam penelitian ini adalah 200 ekor. Eugenol yang digunakan berbentuk cair yang diisolasi dari minyak atsiri daun cengkeh. Perlakuan yang digunakan adalah E0 (Pakan Basal tanpa penambahan eugenol daun cengkeh), E1 (Pakan basal + eugenol daun cengkeh 0,5%), E2 (Pakan basal + eugenol daun cengkeh 1,0%) dan E3 (Pakan basal + eugenol daun cengkeh 1,5%). Parameter yang diukur adalah konsumsi protein, konsumsi energi metabolis, efisiensi penggunaan pakan dan rasio efisiensi protein. Hasil penelitian menunjukkan bahwa penambahan eugenol daun cengkeh 0,5 – 1,5% dalam pakan dapat meningkatkan secara sangat nyata (P<0.01) konsumsi protein dan energi metabolis pakan, efisiensi penggunaan pakan dan rasio efisiensi protein pada ayam pedaging. Efisiensi pakan dan rasio efisiensi protein pakan terbaik pada penggunaan eugenol daun cengkeh 1% dalam pakan ayam pedaging masing-masing 0,62±0,02 dan 3,00±0,12 sehingga disarankan penggunaan eugenol daun cengkeh pada ayam pedaging 1% dalam pakan.