Residue contact map is essential for protein three-dimensional structure determination. But most of the current contact prediction methods based on residue co-evolution suffer from high false-positives as introduced by indirect and transitive contacts (i.e., residues A–B and B–C are in contact, but A–C are not). Built on the work by Feizi et al. (Nat Biotechnol 2013; 31:726–733), which demonstrated a general network model to distinguish direct dependencies by network deconvolution, this study presents a new balanced network deconvolution (BND) algorithm to identify optimized dependency matrix without limit on the eigenvalue range in the applied network systems. The algorithm was used to filter contact predictions of five widely used co-evolution methods. On the test of proteins from three benchmark datasets of the 9th critical assessment of protein structure prediction (CASP9), CASP10, and PSICOV (precise structural contact prediction using sparse inverse covariance estimation) database experiments, the BND can improve the medium- and long-range contact predictions at the L/5 cutoff by 55.59% and 47.68%, respectively, without additional central processing unit cost. The improvement is statistically significant, with a P-value < 5.93 × 10−3 in the Student’s t-test. A further comparison with the ab initio structure predictions in CASPs showed that the usefulness of the current co-evolution-based contact prediction to the three-dimensional structure modeling relies on the number of homologous sequences existing in the sequence databases. BND can be used as a general contact refinement method, which is freely available at: http://www.csbio.sjtu.edu.cn/bioinf/BND/.

Abstract. Ice engraving is a special form of prints. Its medium is ice, while it is inherits traditional painting-creation techniques. Ice engravers are also boldly trying new concepts and methods of expression. Their selection of material has completely stepped out from the traditional ways, which enables it to break free from the restrictions of the environment. Factors such as temperature and time, give it very special characteristics, such as transience, contingency, uniqueness and innovation, which also vividly reflect the regional characteristics of culture and the artistic innovative spirit. The creative process and aesthetic characteristics of ice engravings are valuable for their research significance. This article briefly analyzes and expounds views on these issues...

When the GPS signal or the wireless communication signal is weak, the vehicle will disappear in the map of the control center. In order to solve this problem, an intelligent public transportation terminal system which is cored by S5PV210 microprocessor is designed. The system uses BD2 and GPS dual-mode positioning module. Compass can provide short-message communication service. When the wireless communication signal is weak, we can use this service to communicate with the monitoring center. This design solution will make sure that the vehicle will never disappear in the map of the control center.

Abstract. As a famous monk painter in late Ming and early Qing Dynasties, Dan Dang kept the same fame with those famous painters as Shi Tao (a famous painter in Qing Dynasty)