A new method for the determination of cadmium and lead in human teeth was developed based on dispersive liquid-liquid microextraction preconcentration and graphite furnace atomic absorption spectrometry determination. In the proposed approach, O,O-diethyldithiophosphate (DDTP) was used as a chelating agent, and carbon tetrachloride and methanol were selected as extraction and dispersive solvents. Some factors influencing the extraction efficiency of cadmium and lead and their subsequent determination, including extraction and dispersive solvent type and volume, pH of sample solution, concentration of the chelating agent and extraction time, were studied and optimized. Under the optimum conditions, the enrichment factor of 116 and 68 for cadmium and lead were achieved. The detection limit for cadmium and lead was 5.6 and 45 ng L -1 , and the relative standard deviation (R.S.D) was 4.5% and 3.8% (n = 7, c = 1.0 ng mL -1 ), respectively. Verification of the accuracy of the method was carried out by analysis of a standard reference material (NIST 1486, bone meal). The method was successfully applied to the determination of trace amount of cadmium and lead in human teeth samples with satisfactory results.