The key regulatory roles of circular RNAs (circRNAs) in human diseases have been demonstrated, including breast cancer (BC). The purpose of this study is to explore the role of circ_0102273, a newly discovered circRNA, in BC progression. The expression levels of circ_0102273, microRNA (miR)-1236-3p and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) were determined by quantitative real-time PCR. Cell proliferation, migration and invasion were measured using colony formation assay, EdU staining, wound healing assay and transwell assay. Glucose consumption, lactate production and ATP level were detected to evaluate cell glycolysis. The interaction between miR-1236-3p and circ_0102273 or PFKFB3 was confirmed by dual-luciferase reporter assay and RIP assay. Additionally, western blot analysis was utilized for measuring PFKFB3 protein expression. In-vivo experiments were performed to further explore the function of circ_0102273 in BC tumorigenesis. Our data showed that circ_0102273 was highly expressed in BC tumor tissues and cells, and its downregulation could inhibit BC cell proliferation, metastasis and glycolysis. MiR-1236-3p was confirmed to be sponged by circ_0102273, and its inhibitor could reverse the negative regulation of sh-circ_0102273 on BC cell proliferation, metastasis and glycolysis. PFKFB3 could be targeted by miR-1236-3p, and its expression could be positively regulated by circ_0102273. In addition, miR-1236-3p could suppress BC cell proliferation, metastasis and glycolysis, while this effect could be abolished by PFKFB3. Furthermore, circ_0102273 knockdown also had been discovered to reduce BC tumorigenesis in vivo. In summary, our research proposed that circ_0102273 might be a potential target for BC treatment, which could inhibit BC proliferation, metastasis and glycolysis through the miR-1236-3p/PFKFB3 axis.

IntroductionOne of the plants that has long been considered by humans is Equisetum arvense L Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders.Material and methodsIn the current study, the antioxidant, cytotoxicity, and anti-human ling cancer properties of Equisetum arvense were investigated in the in vitro condition. Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were run to evaluate the antioxidant activity. MTT assay was chosen to investigate anticancer activity of the plant extract.ResultsThe plant extract scavenged DPPH as a free radical with an IC50 of 12.3±0.7 µg/mL better than positive controls. The plant also was rich in phenolic compounds with an amount of 396.2±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of human colorectal over the Equisetum arvense L. The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity on the normal (HUVEC) cell line.ConclusionsThe best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines was in the case of HT 29 cell line.