Background: Platelet Rich Plasma (PRP) is based on the release of growth factors stimulating the initiation/ extension of anagen phase as well as promoting vascularization, Adipose Derived Stem Cell (AT-ADSCs) treatment were recently introduced as an alternative potential therapeutic application for hair growth. Objective: The aim of this study was to assess the efficacy side effects and safety of AT-ASCs and PRP in the treatment of androgentic alopecia. Patients and methods: Sixty randomized patients were treated by PRP, and AT-ASCs. Each patient was evaluated, and each lesion was treated by those modalities, patients received three sessions with one month interval for 3 months, follow up after 3 months. Results: A highly significant improvement <0.001 in terminal hair count of AT-ASCs group evaluated by videodermoscopy assessment of AGA. That were confirmed by highly significant improvement in intermediate hair count and mean caliber (<0.001) associated with high incidence of side effects especially headache and erythema. In contrast, PRP group showed significant improvement 0.037 in terminal hair count and non-significant improvement in intermediate hair count and of mean caliber with minimum side effects. AT-ASCs showed a significant improvement in terminal hair count than PRP, Highly significant improvement in Intermediate hair count and hair caliber. Also, side effects of AT-ASCs showed highly significant pain, headache and erythema but no serious adverse events. Conclusion: Our study suggests that the There was significant improvement in AGA after PRP and highly significant after AT-ASCs therapy with significant difference of ADSC in terminal hair count and highly significant in caliber. Both modalities could effectively and safely be used to treat AGA.

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The aim of this research is to investigate the teratogenic effects of chitosan oligosaccharide in Wistar female rats (Rattus norvegicus). Chitosan LD50 value was calculated by probit analysis. High dose, 1/10 LD50 which equal to 150 mg/kg body weight, and low dose, 1/30 LD50 which equal to 50 mg/kg body weight, were administrated orally to Wistar female rats to examine the teratogenic effect during organogenesis period from 6th day to 15th day of gestation. Treated and control rats were sacrificed and their foetuses were examined for external, skeletal and visceral anomalies, number and length of foetuses and their weights. Obtained results showed toxicity and teratogenic effects of chitosan on treated rats and their progenies, i.e. high fetal mortality, offspring's weight and length reduction, and high incidence of fetal external, skeletal and visceral abnormalities. This suggested that chitosan is a teratogenic compound, restricted to current results from orally treated Wistar rats.

Objective: The aim of this work is to evaluate treatment of vitiligo by co-culture of melanocytes derived from hair follicle with adipose-derived stem cells with (NB-UVB) and without (NB-UVB). Patients and methods:In this study, we used co culture of adipose derived stem cell with melanocytes derived from hair follicle in treating different types of stable resistant vitiligo, by two methods transplantation: group (A) exposed to (NB-UVB), group (B) did not expose to (NB-UVB). They are followed up for 3 months.Results: At the end of the follow up period which was 3 months group (A) showed better pigmentary response than group (B) and it was highly statistically significant. Stability, size, site and onset of vitiligo appeared to be important factors affecting treatment results. Using (NB-UVB) also after injection of the treatment showed more improvement in the treatment results. Conclusion:Co-culture of adipose derived stem cell with melanocytes derived from hair follicle could be a safe and effective method of treatment for stable localized vitiligo in patients resistant to other methods of therapy.

Background The pathogenesis of bronchopulmonary dysplasia (BPD) includes arrest of alveolar septation and enhanced fibrosis. We hypothesized that mesenchymal stromal cells (MSC) and transforming growth factor-β1 (TGF-β1) in tracheal aspirates of mechanically ventilated premature infants differ in BPD and non-BPD infants. Methods Tracheal aspirates were collected during the first week of life. Mononuclear cells were separated, cultured and immunophenotyped by flow cytometry. MSCs colony/cluster ratio was calculated as an index for dysplastic potentials. TGF-β1 was assessed by enzyme-linked immunosorbent assay (ELISA). Setting: Neonatal intensive care unit. Patients Premature infants at risk for BPD. Results A total of 121 preterm infants were enrolled; 27 of them died and among the 94 survivors 23 infants had BPD. MSCs were identified in younger [gestational age (GA): 30.9±1.7 vs. 31.8±1.8, P=0.025] and smaller [birth weight (BW): 1.3±0.28 vs. 1.44±0.37 kg, P=0.04] infants with lower Apgar scores. The recovery rate of MSCs in BPD and non-BPD groups did not differ. BPD group had significantly smaller colony/cluster ratio compared to non-BPD (0.97 vs. 4.25, P=0.002). TGF-β1 was significantly greater in BPD infants (4173.9±864.3 vs. 3705.8±540.5 pg/mL, P=0.021). Conclusion Infants with BPD had different MSCs morphology and greater TGF-β1 expression. The pathogenesis for these morphological changes of resident lung MSCs needs further studying.