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Aim:The aim of our study was polymerase chain reaction (PCR) detection of the genes responsible for the multiple antibiotic resistance S. aureus isolated from food of animal origin in Egypt.Materials and Methods:A total of 125 samples were randomly collected from milk, meat, and their products from Giza and Beni-Suef Governorates markets. The S. aureus isolates were subjected to antimicrobial sensitivity tests using four antibacterial disks (Oxoid), and then the polymerase chain reaction (PCR) was performed for detection of antibiotic resistance genes.Results:Out of 125 samples, 19 S. aureus isolates were detected. All detected isolates were multiple drug resistance (MDR). The penicillin-, erythromycin-, kanamycin-, and tetracycline-resistant isolates were examined by PCR for resistance genes blaZ, (msrA, ermB, and ermC), aac(6’)aph (2”), and tetK. The isolates harbored these resistance genes with percentage of 100% (100%, 0%, and 100%), 62.5%, and 100%, respectively.Conclusion:Contaminated foods of animal origin may represent a source of MDR S. aureus that can be a major threat to public health.

Objective: The objectives of this study were to determine the biofilm-forming capability and antimicrobial susceptibility of Escherichia coli recovered from bovine endometritis samples. Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated E. coli by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes. Results: A total of 58(48.3%) E. coli isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethoxazole- trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all isolates harbored bla TEM , sul 1, tet A, qnr S, bla CTX-M , and fim H with a percentage of 100%, pap C (40%), and hly A (10%). Fim H was the most prevalent biofilm-associated gene. Conclusion: The present study highlights the high prevalence of multi-drug- resistant E. coli associated with bovine endometritis. The detection of the fim H gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic E. coli .

A B S T R A C TThe present study was designed in order to estimate the prevalence of Salmonella spp. in broiler carcasses and human stools in Beni-Suef province (Egypt). Also, the serological identification and testing of the antimicrobial resistance/susceptibility of the isolates have been done. The obtained results revealed that the prevalence of Salmonella in broiler meat, skin, and pooled giblets (liver, gizzard, and heart) was 76, 80, and 64%, respectively, while in the case of human stools the percentage of positive samples represented 4%. The predominant serotype in broiler carcasses was Salmonella Infantis (56.36%) followed by Salmonella Kentucky (25.45%), and then Salmonella Enteritidis with a percentage of 5.45%. However, two serotypes of each of Salmonella Ferruch, Salmonella Kottbus, and Salmonella Virchow were identified out of 55 Salmonella isolates, while the only isolate found in human stool samples was serotyped as Salmonella Infantis. The results of antimicrobial resistance/susceptibility highlighted the existence of multiple antibiotic resistance (MAR) by several strains of Salmonella.