Present study was performed to identify the genetic make-up of thirteen mid-season mango verities i.e.Zardalu, Banganpali, Taimuria, Saleh Bhai, Badia Muna Syed, Intikhab, Anmole, Bombay Alphanso, Ghulab Khas, Pan, Lahotia, Kachnaliwala, and Jo villagey by applying seventy SSR markers, data collected on genetic diversity estimation bifurcated the varieties into three clusters. cluster 1, enclosed three varieties namely Bombay Alphonso, Intikhab, and Anmole together in genetic resemblance. Similarly, the cluster 2 assembled seven varieties i.e. Jo Village, Taimuria, Zardalu, Bagan Palli, Saleh Bhai, Badia and Munna Syed, making it the larger cluster. Lastly, the genetic similarity of the remaining four varieties such as Pan, Ghulab Khas, Lahotia, and Kachnalwala was found in close proximity. The current study singled out three unique fingerprints amongst the seventy SSRs applied. Baganpali cultivar contained unique fingerprints against primer MiKVR_a010; Gulab khas cultivar showcased unique characters against primer MiKVR_a052 and another distinguished fingerprint was observed in mango cv Baganpali against primerMiKVR_a109. It was an indication that these varieties possessed specific genotypic behavior against applied markers. It is concluded that these varieties can play a significant role in improving the genetic makeup of mango varieties and can divulge useful features for varietal selection in a breeding program.
