Present study was performed to identify the genetic make-up of thirteen mid-season mango verities i.e.Zardalu, Banganpali, Taimuria, Saleh Bhai, Badia Muna Syed, Intikhab, Anmole, Bombay Alphanso, Ghulab Khas, Pan, Lahotia, Kachnaliwala, and Jo villagey by applying seventy SSR markers, data collected on genetic diversity estimation bifurcated the varieties into three clusters. cluster 1, enclosed three varieties namely Bombay Alphonso, Intikhab, and Anmole together in genetic resemblance. Similarly, the cluster 2 assembled seven varieties i.e. Jo Village, Taimuria, Zardalu, Bagan Palli, Saleh Bhai, Badia and Munna Syed, making it the larger cluster. Lastly, the genetic similarity of the remaining four varieties such as Pan, Ghulab Khas, Lahotia, and Kachnalwala was found in close proximity. The current study singled out three unique fingerprints amongst the seventy SSRs applied. Baganpali cultivar contained unique fingerprints against primer MiKVR_a010; Gulab khas cultivar showcased unique characters against primer MiKVR_a052 and another distinguished fingerprint was observed in mango cv Baganpali against primerMiKVR_a109. It was an indication that these varieties possessed specific genotypic behavior against applied markers. It is concluded that these varieties can play a significant role in improving the genetic makeup of mango varieties and can divulge useful features for varietal selection in a breeding program.

Present study was performed to identify the genetic make-up of thirteen mid-season mango verities i.e.Zardalu, Banganpali, Taimuria, Saleh Bhai, Badia Muna Syed, Intikhab, Anmole, Bombay Alphanso, Ghulab Khas, Pan, Lahotia, Kachnaliwala, and Jo villagey by applying seventy SSR markers, data collected on genetic diversity estimation bifurcated the varieties into three clusters. cluster 1, enclosed three varieties namely Bombay Alphonso, Intikhab, and Anmole together in genetic resemblance. Similarly, the cluster 2 assembled seven varieties i.e. Jo Village, Taimuria, Zardalu, Bagan Palli, Saleh Bhai, Badia and Munna Syed, making it the larger cluster. Lastly, the genetic similarity of the remaining four varieties such as Pan, Ghulab Khas, Lahotia, and Kachnalwala was found in close proximity. The current study singled out three unique fingerprints amongst the seventy SSRs applied. Baganpali cultivar contained unique fingerprints against primer MiKVR_a010; Gulab khas cultivar showcased unique characters against primer MiKVR_a052 and another distinguished fingerprint was observed in mango cv Baganpali against primerMiKVR_a109. It was an indication that these varieties possessed specific genotypic behavior against applied markers. It is concluded that these varieties can play a significant role in improving the genetic makeup of mango varieties and can divulge useful features for varietal selection in a breeding program.

Genetic diversity estimation of 13 varieties of mango was assessed using 28 microsatellite markers. Mean values of 3.9231, 0.4455, 0.5407 and 0.4952 were observed for alleles, minor alleles frequency, gene diversity and polymorphic information content respectively. The observed heterozygosity value of YY implies that spontaneous hybridization may have contributed to the ancestry of some genotypes and that improvement by farmers may have possibly been by selection of somatic mutants. The dendrogram of the cluster analysis revealed three groups. Findings demonstrated the efficiency of SSR markers in distinguishing closely related mango varieties due to their high specificity. The study contributes to the understanding of the genetic organization of the mango germplasm. Nonetheless, fifteen SSR markers were used in thirteen exotic mango varieties to calculate polymorphism percentage, genetic diversity, frequency based genetic distances, genetic diversity percentage, allelic frequencies and PIC values. Polymorphism percentage recorded was 76.48%. Moreover, varietal similarity index of genome helped to construct phylogenetic tree that divided all varieties into three clusters according to their genetic similarities. First cluster that exhibited similarity in genetic consonance included varieties viz. Collector, Haden, Zill, Early gold and Pope. Two varieties i.e. Momi-k and Spring Fells were similar to each other genetically. Similarly, third cluster encapsulated Kensington Pride, Tomi-Atkin, Keitt, Maya and Sensation cultivars in genetic harmony.