The aim of the present study was to evaluate hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in mussels (Mytilus galloprovincialis) from Cherrat estuary (Moroccan Atlantic Coast), Morocco. In total, 52 samples (n = 12 mussels/each) were collected at four sites in the estuary, monthly, between March 2019 and March 2020. HAV and HEV were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) according to the ISO/TS 15216 method. HAV was detected in 46.15% of analyzed samples. Conversely, HEV was not detected in any sample. Moreover, the HAV detection rate was significantly associated with seasonal rainfall variations. This qualitative study on HAV and HEV contamination highlights the interest of studying mussel samples from wild areas. As HAV presence in mussels represents a potential health risk, viral contamination surveillance of mussels is necessary to protect consumers. HAV shellfish contamination must be monitored at Cherrat estuary because of the role played by shellfish as HAV reservoirs and/or vehicles in fecal-oral HAV transmission.

No abstract

No abstract

Mussels filter large amounts of water to extract nutrients; therefore, they can concentrate and accumulate in their tissues infectious agents, and vectors of enteric diseases. The aim of this study was to assess mussel contamination by Norovirus genogroups I and II  in the Cherrat estuary to determine the public health risk linked to their consumption. Mussels (Mytilus galloprovincialis) were collected (n=52 samples; 12 mussels/sample) at four sites (S1 and S2 located on the right rocky bank / S3 and S4, located on the rocky left bank) in the Cherrat estuary (Casa-Settat region), Morocco, during 13 months, from March 2019 to March 2020. Norovirus was detected and quantified by real-time Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Norovirus genogroups I and II were detected in 17.30% and 94.23% of mussel samples, respectively. Contamination by Norovirus (genogroups I and II) was not correlated with seasonal factors (month and rainfall), and Norovirus prevalence was comparable among the four sampling sites. Consumption of raw or undercooked mussels contaminated with Norovirus can cause gastroenteritis, which represents a potential risk to human health. The present study would be helpful to control and manage the potential risk to the public health of the Moroccan population due to the degradation of water quality continuously impacted by runoff, the urban wastewater treatment system malfunctions, and overflows from nearby sewage systems.