This study aims to determine the effect of Edmodo-based E-learning on the participation and understanding of elementary school teacher candidate in science 1 biology courses. This study used a quasi-experimental method using a non-equivalent control group design. The sample of this study was 49 elementary school teacher candidates in semester 2 of PGRI Yogyakarta University. The data collection technique used a participatory questionnaire instrument and a pretest-posttest comprehension test. The results of the Independent Sample t-Test show that the value of Sig. for the participation variable of 0.004 <0.05 and the Sig. for the understanding variable of 0.005 <0.05. While, the MANOVA test results show the value of Sig. for the participation variable of 0.004 <0.05 and the Sig. for the understanding variable of 0.005 <0.05. This means that there are differences in the increase in participation and understanding of elementary school teacher candidate after using Edmodo-based Elearning. In addition, the application of Edmodo-based E-learning also has a positive effect on the participation and understanding of elementary school teacher candidate on science topics. Edmodo-based e-learning makes elementary school teacher candidates more active in participating and easier in understanding science lecture material 1.

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