Um método empregando LC-MS/MS foi desenvolvido para a análise de rosuvastatina em plasma humano, usando atorvastatina como padrão interno. Rosuvastatina é um fármaco para redução de lipídeos e prescrita para o tratamento do hipercolesterolemia e de dislipidimia. A extração em fase sólida (SPE) foi usada para purificação e pré-concentração do analito a partir da matriz do plasma humano. A separação cromatográfica foi conseguida em 6.0 min empregando fase móvel composta de 0.2% de ácido fórmico em água e acetonitrila (40: 60,v/v, na vazão de 1.0 mL min -1 e coluna YMC J'sphere ODS H-80, 150 x 4.6 mm, partículas de 4.0 μm. Na saída da coluna, a fase móvel foi dividida, sendo que 200 μL foram dirigidos para o espectrômetro de massas e 800 μL para o descarte. Pelo Monitoramento de Reação Múltipla (MRM), as transições foram medidas no modo positivo em m/z 482 -258 para rosuvastatina e m/z 559 -440 para o padrão interno, respectivamente. Uma validação detalhada do método foi realizada seguindo as recomendações do FDA americano e as curvas analíticas foram lineares no intervalo de 1.00 ng mL -1 a 50.00 ng mL -1 com coeficiente de correlação médio maior que 0.99. A recuperação absoluta foi maior que 50.14% para rosuvastatina e 54.65% para o padrão interno. Rosuvastatina foi estável por 138 dias a -70 ± 5 °C e por 24 horas à temperatura ambiente. Após a extração do plasma, as amostras reconstituídas de rosuvastatina permaneceram estáveis no auto injetor, a 10 °C, por 8 horas. Depois de submetidas a três ciclos de congelamento/descongelamento, não houve mudanças na recuperação do analito. O método é simples, específico, sensível, preciso, exato e apropriado para aplicações em bioequivalência e estudos farmacocinéticos. Foi aplicado com sucesso em um estudo piloto de bioequivalência da rosuvastatina, comprimidos -Zydus, Cadila, India versus comprimidos -Crestor, Astra Zeneca, EUA, em voluntários sadios do sexo masculino.A LC-MS/MS method has been developed for the estimation of rosuvastatin in human plasma using atorvastatin as internal standard. Rosuvastatin is a lipid-lowering drug prescribed for the treatment of hyper-cholestrolemia and dyslipidimia. Solid phase extraction (SPE) was used for the purification and pre-concentration of analyte from human plasma matrix. The chromatographic separation was achieved within 6.0 min by an isocratic mobile phase containing 0.2% formic acid in water and acetonitrile (40: 60, v/v), flowing through YMC J' Sphere ODS H-80, 150 x 4.6 mm, 4.0 μm analytical column, at a flow rate of 1.0 mL min -1 with split of 200 μL to mass spectrometer and 800 μL to waste. Multiple reaction monitoring (MRM) transitions were measured in the positive mode at m/z 482 and 258 for rosuvastatin and m/z 559 and 440 for internal standard respectively. A detailed validation of the method was performed as per USFDA guidelines and the standard curves were found to be linear in the range 1.0 ng mL -1 to 50.0 ng mL -1 with the mean correlation coefficient more than 0.99. The absolute recovery was more than 50.14% f...

Finally, the validated method was successfully applied to bioequivalence studies.

No abstract

In recent days, Whenever someone wants to take a book from library he or she has to go through all the books available in library related to the particular subject. Even if the book is not available in that library, the user will have to search the entire department. Also the manual work done by the librarians is tedious as they have to organise the books as per the department and also have to maintain the records To summarise the present day library system is not efficient and need improvement with the advance technology . To solve the problem of manual work done in the present day library system, we propose the idea of using a smart cart for accessing the library catalogue. It will automate the process of borrowing a book or issuing a book or any other literature available in library. We propose to use an intelligent system that reduces the manual work and fastens the processes in a library. Also we can merge the data- bases of various public libraries available in a particular city and provide the information to the citizens so that it will be easier for them to go to only that specific library which has the book instead of browsing through all the libraries and moving from place to place .

A novel High Performance Liquid Chromatography-electrospray mass spectrometric method has been developed for the estimation of Ursodiol (Ursodeoxycholic acid)--a bile acid, in human plasma using Ornidazole as internal standard. The methodology involved solid phase extraction of the analyte from human plasma matrix. The chromatographic separation was achieved within seven minutes by an isocratic mobile phase containing 1.0 mM ammonium acetate and Acetonitrile (65:35, v/v), flowing through XTerra MS C18, 100 x 2.1, 3.5 microm analytical column, at a flow rate of 0.2 ml/min. Ion signals were measured in negative mode for Ursodiol and internal standard at m/z 391.3 and 278.1, respectively. A detailed validation of the method was performed as per USFDA guidelines and the standard curves were found to be linear in the range 50.0 ng/ml to 3000.0 ng/ml with the mean correlation coefficient more than 0.99. The absolute recovery was more than 54.90% for Ursodiol and 76.51% for internal standard. Ursodiol was stable for sixty-nine days at -70 degrees C and for eight hours at ambient temperature. After extraction from plasma, the reconstituted samples of Ursodiol were stable in autosampler at 10 degrees C for forty-eight hours. Upon subjecting to three freeze thaw cycles, there was no change in the recovery of the analyte. The integrity of the plasma samples remained unaffected even upon four-fold dilution with drug free human plasma. The method was simple, specific, sensitive, precise, accurate and suitable for bioequivalence and pharmacokinetic studies. It was successfully applied to the pilot bioequivalence study of Ursodiol in male human subjects.