Inula viscosa is a type of Asteraceae family, which widely used for medicinal plant to treatment of different diseases. Inula viscosa contains many biological compounds, such as monoterpenes, sesquiterpenes, diterpenes, flavonoids. Our study aimed to investigate the cytotoxic effects of Inula viscosa extract on MCF-7 (breast carcinoma), C6 (glioblostoma cancer), MG63 (bone osteosarcoma), cancer cells and L929 (mouse fibroblostama) cell lines. Cytotoxic effects of the Inula viscosa extracts were performed by XTT assay. In this study, Inula viscosa extracts showed selective cytotoxic effect on MC-7 compared than L929 cells. The Inula viscosa extracts appear to be a promising source of new anticancer agent. Further studies are needed to identify the cytotoxic activity mechanisms on these cancer cell lines.

AbstractThis study was aimed to determine the effect of different boron doses (boron free, pure boron with 8 liters per decare and in 1/8 ratio diluted boron) on the rate and quality of volatile oil in musk grown in Kütahya-Gediz conditions. Essential oil from Musk Sage was obtained by hydrodistillation method (GC-MS/FID). In the analysis carried out in 2017, the rate of essential oil was found to be 0.11% in the plant grown without boron, while it was 0.44% in the plant grown with pure boron. However, this rate was found as 0.23% in the 1/8 boron ratio application. The main components of volatile oil were found as follows: for the boron-free application—spathulenol 23.75%, caryophyllene oxide 19.41%, linalool 10.10%, and sclareoloxide 9.92%; for the pure dose application—spathulenol 26.67%, sclareoloxide 18.81%, and caryophyllene oxide 16.13%; for in 1/8 ratio diluted boron dose application—spathulenol 24.82%, sclareoloxide 16.68%, and caryophyllene oxide 14.86%. It has been observed that pure boron dose has a positive effect on the essential oil ratio and components of Musk Sage.

Bu araştırma Kütahya (Gediz) yöresinde yetiştirilen Salvia officinalis L. (Lamiaceae) türünün uçucu yağ bileşenleri, uçucu yağ oranı ve kalitesi üzerine etkisini belirlemek amacıyla yürütülmüştür. Salvia officinalis L. türünün tohum ve yaprağında uçucu yağ analizi yapılmıştır. Bu türün uçucu yağı hidrodestilasyon (GC_MS/FID) yoluyla elde edilmiştir. Uçucu yağ oranı yıllara (2016-2017) göre yaprakta birinci ve ikinci yıl %2.08-2.23, ikinci yıl tohumda %0.08 olarak ölçülmüştür Yapılan analiz sonucunda tohumda toplam 14 bileşen, yaprakta ise 17 bileşen tespit edilmiştir. Tohumda elde edilen uçucu yağdaki ana bileşenler, viridiflorol %22.01, α-thujone %21.46, Borneol %15.03, β-thujone %6.93, 1.8-cineole %6.92 olarak tespit edilmiştir. Yaprakta elde edilen uçucu yağdaki ana bileşenler, αthujone %31.82, 1.8-cineole %14.39, camphor %12.05, β-thujone %8.55 olarak bulunmuştur. Tohum ve yaprakta yapılan analiz sonucunda ortak bileşen olarak; viridiflorol, α-thujone, β-thujone, 1.8-cineole, βcaryophyhllene, α-humulene, borneol olarak tespit edilmiştir. Yapılan çalışma sonunda; uçucu yağ bileşenlerinin terpenler bakımından zengin olduğu belirlenmiştir.

This study was conducted simultaneously in different locations in Çanakkale, Balıkesir and Kütahya in order to determine the effect of location on the volatile oil components, volatile oil rate and volatile oil quality of Abyssinian sage, Musk sage and Medical sage (Salvia aethiopis L., Salvia sclarea L. and Salvia officinalis L. (hybrid)) plants from the 2015 growing season. Field experiments were carried out in 3 replicates according to the randomized block design. These plants’ volatile oils were obtained by the hydrodistillation method (GC-MS/FID) and the volatile oil rates in three different locations were measured as 0.53%, 0.21%, 0.20%, respectively. The basic components of the volatile oil were determined as follows: β-caryophyllene 36.22%, 30.46%, 35.96%, α-copaene 15.06%, 16.46%, 16.58%, germacrene-D 13.23%, 20.01%, 15.20%, β-cubebene 5.62%, 7.04%, 6.93%, α–humulene 8.68%, 7.40%, 8.54%, caryophylleneoxide 7.40%, 1.82%, 3.53%. No volatile oil was acquired from Salvia sclarea L. except for the Çanakkale location which was only 0.02% and the main components in this volatile oil were measured as germacrene-D 20.78%, and phytol 17.81%. The best volatile oil contents from Abyssinian sage and Musk sage were obtained from the Çanakkale location with 0.53% and 0.02%, respectively. The rates of volatile oils from Medical sage (Salvia officinalis L. (hybrid)) were 1.00%, 1.40% and 0.96%, respectively, in the three locations. The main components in this volatile oil were measured as α-thujone 46,00%, 44.53%, 35.78%, β-thujone 5.05%, 6.31%, 8.61%, camphor 10.73%, 19.15%, 18.68%, 1.8-cineole 8.99%, 7.23%, 5.06%, viridiflorol 1.85%, 2.28%, 4.23%. The highest volatile oil rate in Medical sage was reached at the Balıkesir location at a rate of 1.40%. As a result of this study it was found that volatile oil components are comparatively richer in terpenes and the amount of volatile oil differs according to ecological factors.

ÖZET: Rezene (Foeniculum vulgare Mill.) ticari olarak üretimi yapılan önemli baharat bitkilerindendir. Rezene; gaz giderici özelliğe sahip olmasının yanında ayrıca koku, tat ve uçucu yağ bakımından zengindir ve ayrıca baharat olarak yaygın kullanıma sahip bir bitkidir. Bu çalışma, Kütahya -Gediz koşullarında farklı bor dozlarının (0, Saf doz, 1/8 dozda dekara 8 lt) Rezene (Foeniculum vulgare Mill.)'de bulunan uçucu yağ oranı ve kalitesi üzerine etkisini belirlemek amacıyla, 2017 yılında Dumlupınar Üniversitesi, Tıbbi ve Aromatik Bitkiler deneme tarlasında yürütülmüştür. 2017 yılında tarla denemeleri tesadüf blokları deneme desenine göre 3 tekerrürlü olarak kurulmuştur. Uçucu yağ bileşimi Gaz Kromatografi-Kütle Spektrometresi (GC-MS) ile belirlenmiştir. Uçucu yağ oranları sırasıyla borsuz doz %1.83, saf doz %3.43, 1/8 dozda %1.55 olarak bulunmuştur. Borsuz, saf dozda ve 1/8 dozda yetişen rezenelerin meyvelerinde yapılan 3 farklı dozun analiz sonucunda 7'şer bileşen tespit edilmiştir. Borsuz çalışmada elde edilen uçucu yağdaki ana bileşen sırasıyla; trans-anetol %84.65, limonen %5.92, pallilanisol %4.18, fenkon %1.12'dir. Saf dozda elde edilen bileşenler ise; trans-anetol %94.52, anisol %3.54, limonen %1.09, fenkon %0.36 olarak bulunurken, 1/8 dozda trans-anetol %92.38, anisole %3.8, limonen %2.85, fenkon %0.35 olarak bulunmuştur.