SummaryHygienic behaviour in the honey bee, Apis mellifera, is the uncapping and removal of dead, diseased or infected brood from sealed cells by worker bees. We determined the effect of hygienic behaviour on varroa population growth and incidence of deformed wing virus (DWV), which can be transmitted by varroa. We treated 42 broodless honey bee colonies with oxalic acid in early January 2013 to reduce varroa populations to low levels, which we quantified by extracting mites from a sample of worker bees. We quantified varroa levels, again when the colonies were broodless, 48 weeks later. During the summer the hygienic behaviour in each colony was quantified four times using the Freeze Killed Brood (FKB) removal assay, and ranged from 27.5 % to 100 %. Varroa population increased greatly over the season, and there was a significant negative correlation between varroa increase and FKB removal. This was entirely due to fully hygienic colonies with >95 % FKB having only 43 % of the varroa build up of the less hygienic colonies. None of the 14 colonies with >80 % FKB removal had overt symptoms of DWV, whilst 36 % of the less hygienic colonies did. Higher levels of FKB removal also correlated significantly with lower numbers of DWV RNA copies in worker bees, but not in varroa mites. On average, fully hygienic colonies had c. 10,000 times less viral RNA than less hygienic colonies. Hacia el control integrado de varroa: efecto de la variación en el comportamiento higiénico entre colonias de abejas de la miel con aumento de la población de ácaros y de la incidencia de virus de las alas deformadas ResumenEl comportamiento higiénico en la abeja de la miel, Apis mellifera, se basa en el desoperculado y la eliminación de la cría muerta, enferma o infectada a de las celdas selladas por las abejas obreras. Se determinó el efecto del comportamiento higiénico en el crecimiento de la población de varroa y la incidencia del virus de las alas deformadas (VAD), que puede ser transmitido por la varroa. Se han tratado 42 colonias de abejas de la miel sin larvas con ácido oxálico a principios de enero de 2013 para reducir las poblaciones de varroa a niveles bajos, lo que fue cuantificado mediante la extracción de los ácaros de una muestra de las abejas obreras. Se cuantificaron los niveles de varroa, de nuevo cuando las colonias no tenían cría, 48 semanas después. Durante el verano, el comportamiento higiénico en cada colonia se cuantificó cuatro veces utilizando el ensayo de congelar la cría para matarla (BCM), y este varió entre el 27,5% y el 100%. La población de Varroa aumentó considerablemente durante la temporada, y se observó una correlación negativa significativa entre el aumento de la varroasis y la 556 Toufailia et al.
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