There are several viruses considered to be ecologically problematic, including COVID-19.The samples were collected from persons with middle and severe cases of patients with COVID-19 as well as other samples from patients who were appeared symptoms or recovered from infection of coronavirus, where the number of cases 100 and measured levels of cytokines in the serum of patients as IL-6, IL-8, and tumor necrosis factor-alpha (TNF-α), estimated by immune assay called enzyme-linked immunosorbent assay (ELISA). Where serum level of TNF-α in persons not illustrated a significant difference in all groups, while the interleukin 6 in severe and middle case groups of COVID-19 was high significantly compared with normal and recovered cases groups of COVID-19. Also, the concentration of interleukin 8 was low in the serum of patients infected by a coronavirus in the cases of severe and middle respectively compared with normal cases and recovered cases that healing from coronavirus infection which maybe gives a prediction for disease COVID-19, also showing different concentrations of cytokines in cases of the COVID-19 group than the normal group that may belong to several mechanisms of immunoregulatory detected in cases of COVID-19 pathogenesis.