Wheat and barley are the most important agricultural crops in many countries around the world, just behind rice and maize in importance. Hence, two field experiments with design of spit plot were carried out in winter season of 2014-2015 at the Faculty of Agriculture Research Farm, Mansoura University, Egypt to investigate total uptake of N, P and K by wheat (Sakha 68) and barley (Gemeza123) under different application rates of mineral and organic fertilizers, as well as grain Protein yield of Kg fed-1. Treatments of organic were chicken manure, compost and biochar, while the studied mineral fertilizer treatments took with rates of 50,75,100 and 125 % .The results revealed that total removed (uptake) of NPK by wheat plant were 80.88, 15.79, and 123.54, respectively; while with barley plant they amounted by 61.60, 11.21, and 56.83 were assigned with the treatment of chicken manure + 125% of recommended NPK treatments. Also, the best interaction was found between chicken manure + 125% mineral treatments which gave the highest value of protein uptake which were 310.60 and 278.76 kg fed-1 compared with other interactions of wheat and barley crops. It can conclude that Chicken manure + 125% mineral treatments were the superior in most amendments and gave the highest values of total NPK uptake and protein yield which in turn lead to improve crop productivity as well as sustain soil health and fertility.