No abstract

Genetic linkage maps are valuable tools for genetic, genomic, and crop breeding studies. Several genetic linkage maps were constructed for the olive (Olea europaea L.) genome, mainly using amplified fragment length polymorphisms (AFLPs) and simple sequence repeat (SSR) markers. However, AFLPs and SSR markers were not enough to develop a high-density olive linkage map. Genotyping-by-sequencing (GBS), a recently developed single-nucleotide polymorphism (SNP) identification methodology based on next-generation sequencing (NGS) technologies, has been demonstrated to be useful for the identification of a high number of SNP markers and the construction of high-density genetic linkage maps. In the present study, we identified a total of 10,941 SNPs from a cross between the olive cultivars 'Gemlik' and 'Edincik Su' using GBS and de novo SNP discovery implemented in the computer program "Stacks." A high-density genetic linkage map for the olive genome was constructed using 121 cross-pollinated full-sib F1 progeny and 5643 markers (21 SSRs, 203 AFLPs, and 5736 SNPs). This linkage map was composed of 25 linkage groups, covering 3049 cM of the olive genome, and the mean distance between the flanking markers was 0.53 cM. To the best of our knowledge, this map is the most saturated genetic linkage map in olive to date. We demonstrated that GBS is a valuable tool for the identification of thousands of SNPs for the construction of a saturated genetic linkage map in olive. The high-density genetic map developed in this study is a useful tool for locating quantitative trait loci and other economically important traits in the olive genome.

1 Uludag Uni ver sity, Fac ulty of Ag ri cul ture, De part ment of Hor ti cul ture, Ab stractEf fects of salt stress on the ac tiv ity of peroxidase (PRX) isozyme and leaf pro teins were stud ied in the three straw berry va ri et ies, Camarosa, Tioga and Chan dler. Plants were wa tered with mod i fied 1/3 Hoagland nu tri ent so lu tion con tain ing 0 (con trol), 8.5, 17.0 and 34.0 mM NaCl for 30 days fallowing 20 days ac cli ma tion. PRX ac tiv ity was as sayed in leaf ex tracts and PRX pro files of con trol and salt treated plants were performed by na tive polyacrylamide gel elec tro pho re sis (PAGE).Dur ing the salt stress Tioga and Camarosa tol er ated the cel lu lar dam age with less elec tro lyte leak age while Chandler ex hib ited a se vere cel lu lar dam age with the high est (87.5 % in 34.0 mM NaCl treat ment) elec tro lyte leak age. To tal sol uble pro tein con tent was de creased by sa lin ity in Tioga (from 11.35 to 5.86 mg·g -1 DW) and Chan dler (from 9.35 to 3.90 mg·g -1 DW) while it was al most un changed in Camarosa. Salt stress in creased to tal and spe cific PRX ac tiv ity as com pared with the con trol in all the va ri et ies. In na tive PAGE, one ba sic isoperoxidase band (Rf=0.27) was ob served com monly with dif fer ent band in ten sity in all the treat ments. There fore, it might be as so ci ated with lig ni fi ca tion and re cov ery of cell mem brane dam age in straw berry plants un der salt stress.

No abstract

No abstract