High total blood cholesterol levels are one of the factors that cause atherosclerosis and coronary heart disease. Fruitghurt is a probiotic product made from fruit juice. The content of potential ingredients in red guava and the role of LAB bacteria make fruitghurt a good and useful potential food. This study aims to determine the effect of red guava juice fruitghurt on total blood cholesterol in experimental animals induced by a high-fat diet. This research is a pure laboratory experiment. In this study, 5 different treatments were carried out on 5 groups of experimental animals. The data obtained were tested with several levels of statistical tests, to see the significance of the results. From the results obtained, it was concluded that the one that had a significant effect on reducing total blood cholesterol levels in rats was treatment group 3 given simvastatin and fruitghurt drugs with cholesterol levels reaching 49.54 mg/dl. The decrease in total blood cholesterol levels may be due to the mechanism of action of simvastatin and fruitghurt drugs. The mechanism of action of the drug simvastatin is to competitively inhibit 3-hydroxy-3-methylglutaroyl-coenzyme A causing a decrease in cholesterol synthesis, causing a decrease in total cholesterol. Giving fruitghurt can reduce total cholesterol levels caused by the mechanism of action of probiotics which can increase the secretion of bile salt hydrolase enzymes and produce dehydrogenated cholesterol cofactors so that total cholesterol will decrease. The results of this study indicate that guava juice fruitghurt can reduce blood cholesterol levels in experimental animals.