There have been series of advocacies all over the world to prepare future scientists who would take up the challenge of eradicating environmental pollution through the deployment of scientific knowledge such as green chemistry. This study investigated teachers’ knowledge of green chemistry in Kwara State, Nigeria. The Green Chemistry Knowledge Questionnaire (GCKQ) was used to collect the data. Answers were provided to the research questions using the mean and percentage, while the research hypotheses were subjected to t-Test and ANOVA. Findings from the study revealed that the teachers’ knowledge of green chemistry was high and significantly higher among experienced teachers. It is recommended that the chemistry teachers should endeavor to pass green chemistry ideas to the students and that the experienced chemistry teachers should carry the less experienced teachers along in the course of passing across the knowledge of green chemistry.