Wound infection is described as the occurrence of microorganisms in sufficient numbers or with sufficient virulence in a wound environment to stimulate an immune system response locally, systemically, or both. The present study focused on assessing the immune response by measuring cytokine levels ( IL-1, IL-22 ) in the serum of rats that induced wound infected with Pseudomonas aeruginosa. Thirty-six female Albino rats were used, divided into 3 groups GI (infected group), GII (wound without infection group), and GIII (healthy control group). An excision wound was made on the rat’s back and then contaminated the wound region with a bacterial solution that contain 2.5 ×106 CFU of P. aeruginosa. Following that, blood samples were taken at predetermined time periods (24 hours, 48 hours, and 72 hours) following infection to evaluate immunological response during wound infection. We found that the cytokines (IL-1) were detectable at all times ( 24, 48, 72 ) hours and observed in all studied groups while a significantly elevated level of IL-22 ( 0.77 ± 0.08 ) pg/ml was recorded in 24 hours in the infection group and then decreased at 72 hours.